Combination of these processes would be an ideal, integrated-effective green cutting, mechanical cutting of the future direction of the mainstream.

这些工艺的有机结合将是一种理想的、综合效益好的绿色切削，是未来机械切削加工的主流方向。

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In ultrasonic cutting process, the cutting pressure is still essential for achieving good quality cut edge compared with traditional mechanical cutting process.

在超声切割过程中，为了保证切割质量，对切割压力仍有一定的要求。

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Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.

方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。

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Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.

方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。

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