Objective To test the expression of melanoma antigen-4 (MAGE-4) gene in human colonic adenocarcinoma tissue.
目的检测黑色素瘤抗原- 4 (MAGE - 4)基因在结肠腺癌中的表达情况。
Analyze MAGE-4 antigen epitope by online predict software of protein secondary structure and amplify the epitope gene by PCR.
在线用蛋白质二级结构预测软件分析MAGE - 4抗原表位，PCR获取MAGE - 4抗原特征表位基因。
Conclusion the prokaryotic expression vector for HSP70 and MAGE-4 epitope genes was successfully constructed, which provided a basis for the development of vaccine.
结论已成功构建了人hsp70与MAGE - 4抗原表位基因的原核表达载体，为疫苗研究提供了依据。
Conclusion Specific expression of MAGE-4 gene was proved in human colonic adenocarcinoma tissue, which indicated that MAGE-4 gene might be used for the development of colonic carcinoma vaccine.
结论MAGE - 4基因在结肠腺癌组织中呈特异性表达，提示该基因可用于结肠癌疫苗的开发。
Conclusion Specific expression of MAGE-4 gene was proved in human colonic adenocarcinoma tissue, which indicated that MAGE-4 gene might be used for the development of colonic carcinoma vaccine.
结论MAGE - 4基因在结肠腺癌组织中呈特异性表达，提示该基因可用于结肠癌疫苗的开发。
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