Hepatic stellate cell was isolated with collagenase perfusion and ladder density centrifuge. Hepatic stellate cell line IG12 was cloned with limited diluted method.
利用胶原酶灌注梯密度离心分离肝星状细胞，用有限稀释法建立大鼠肝星状细胞株ig12。
Hepatocytes were isolated by the digitonin-collagenase perfusion technique.
肝细胞通过毛地黄皂苷-胶原酶灌流技术分离。
Methods: The perfusion model of isolated rat liver was established.
方法：建立离体鼠肝灌流模型。
Objective to observe the effect of hypertonic saline solution perfusion on the function of isolated hearts of burned rats.
目的观察高渗盐液对烧伤后大鼠心脏功能的影响。
Objective:To investigate the effect of Astragalus Extractum on canine isolated kidney during hypothermia perfusion and preservation.
目的 ：研究黄芪提取液对犬离体肾低温灌注及保存中缺血再灌注损伤的影响。
Objective: To prolong survival time of the isolated rabbit heart by improving perfusion experiment for isolated rabbit hearts.
目的：通过改进离体心脏灌流试验，延长家兔离体心脏存活时间。
Objective: To prolong survival time of the isolated rabbit heart by improving perfusion experiment for isolated rabbit hearts.
目的：通过改进离体心脏灌流试验，延长家兔离体心脏存活时间。
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