The indirect-ELISA assay was confirmed to have good reproducibility and specificity.
此间接ELISA方法的特异性强、重复性好。
The specificity of phages combined with CTGF was identified by direct and indirect-ELISA.
用直接和间接ELISA方法检测噬菌体阳性克隆与CTGF结合的特异性。
The biologic activity of the expressed product was detected with indirect ELISA.
并用间接ELISA法初步检测表达产物的生物活性。
Methods ANCA fluorescent patterns and its target antigens in sera of 205 patients with renal diseases were determined by indirect immunofluorescence (IIF) and ELISA.
方法应用间接免疫荧光(IIF)和ELISA方法检测了205例肾脏疾病患者血清中的ANCA及其靶抗原。
The methodology of indirect ELISA for the detection of rotavirus (RV) serum antibody has been investigated.
本文对ELISA间接法检测人轮状病毒（RV）血清抗体的方法学进行了探讨。
Objective To screen parathion residues in importing and exporting plants through indirect competitive ELISA method.
目的采用间接竞争ELISA法对进出口植物产品中对硫磷残留量进行快速筛选。
Methods ELISA indirect method, we detected lipopolysacchride core types of E. coli using absorbed lipopolysacchride core type monospecific antisera in E.
方法ELISA间接法，用吸收后单一核心型特异性抗血清检测鉴定E 。
Methods: the mice were immunize with LOVO, and the mAb were screened by indirect cell ELISA and immunohistochemistry.
方法：用人结肠癌细胞株lovo免疫小鼠，用间接细胞ELISA和免疫组化的方法筛选抗结肠癌相关抗原的单抗。
An indirect enzyme-linked immunosorbent assay (ELISA) was developed based on a purified recombinant F41 pili protein of enterotoxigenic Escherichia coli (ETEC).
以纯化的重组F41菌毛蛋白作为检测抗原，建立了检测产肠毒素大肠杆菌F41菌毛抗体的间接ELISA方法。
We utilized recombinant antigen and developed an indirect ELISA assay for detecting PRV antibodies.
本研究应用重组抗原，成功建立了检测猪伪狂犬病病毒血清抗体的间接ELISA诊断方法。
The titer of the antiserum was respectively 1:256 by agarose double-diffusion analysis and it was 1:12800 by indirect ELISA, specific antisera against the fusion protein were prepared.
琼脂糖双向扩散检测显示抗血清的效价1：25 6，间接ELISA测得的效价1：12 800，结果显示获得了融合蛋白的特异性抗血清。
The result was consistent with that of the indirect sandwich ELISA and inhibition ELISA test.
与间接夹心elisa法和抑制酶联免疫吸附试验的结果是一致的。
Thus, indirect ELISA proved to possess high sensitivity, specificity and reproducibility, it can be used to replace indirect sandwich ELISA for the detection of serum antibody against RV.
因此，ELISA间接法敏感性高、特异性强和重复性好，可代替ELISA间接双抗体夹心法以检测RV血清抗体。
Developping indirect ELISA method and determining the blood serum antibody titre.
采血，建立间接ELISA方法测定血清抗体效价。
The egg yolk antibodies were examined with indirect ELISA assay that has been set up, and testified it was against Cryptosporidium.
采用间接ELISA法对获得的卵黄抗体进行初步鉴定，其具有隐孢子虫属特异性。
Methods: Use indirect competitive ELISA method to detect kanamycin, and verify the method's recovery, linearity, specificity, precision, durability.
方法：采用间接竞争ELISA方法检测卡那霉素，并对方法的回收率、线性、特异性、精密性、耐用性等进行验证。
An indirect ELISA was established with expressed 3a protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera, immune sera and other disease sera.
用表达的口蹄疫3a蛋白作为包被抗原，建立了间接elisa试验，对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。
An indirect ELISA was developed and optimized with the purified recombinant E2 protein as coating antigen. The optimal conditions of ELISA were determined as follows.
将表达的重组E2蛋白纯化后作为ELISA包被抗原，建立了一种基于重组E2蛋白的间接ELISA方法，并对此方法的反应条件进行了优化。
The results showed that the positive detectable rate of capture ELISA and indirect N-ELISA is high than IDEXX ELISA kit.
初步结果表明，单抗捕捉ELISA和间接N-ELISA的阳性检出率均比IDEXX试剂盒的高。
We worked on equine rhinopneumonitis serological investigation in Xinjiang Uygor Autonomous Region both with this indirect ELISA and CFT(Complement Fixation Test).
用建立的间接ELISA法和补体结合试验对我区的9个地州作了马 鼻肺炎血清学调查。
OA-OVA, as the coating antigen, were prepared by activated ester method, and at last we established the indirect competitive ELISA for detecting OA.
依照活性酯法将OA与OVA偶联作为包被抗原，建立了间接竞争ELISA检测OA的方法。
Piglets of each groups were vaccinated respectively with recombinant protein vaccine, commodity vaccine and PBS as contrast. The serum IgG anti-protein of each groups were detected by indirect ELISA.
分别以重组蛋白疫苗、商品疫苗和PBS免疫仔猪，优化检测免疫抗体水平的间接ELISA条件，通过比较三个免疫组的抗体水平来评价表达蛋白作为免疫抗原的有效性。
Methods The mice were immunized by different doses of cholera vaccine. Titer of antiserum against cholera strain and toxin were tested by indirect enzyme-linked immunosorbent assay (ELISA).
方法以不同剂量霍乱疫苗免疫小鼠，用间接elisa方法检测小鼠全血的抗菌、抗毒抗体滴度，小鼠毒菌攻击法计算保护率。
An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for cypermethrin and cyhalothrin was developed and optimized by using polyclonal antibodies.
利用自制抗体建立并优化了氯氰菊酯和三氟氯氰菊酯的间接竞争ELISA (IC - ELISA)检测方法。
The method of indirect ELISA to detect CDV antibody in blood serum was established, using recombinant nucleocapsid protein as envelope antigen.
将表达的融合蛋白作为包被抗原，建立了间接酶联免疫吸附试验(间接elisa)的诊断方法以检测CDV血清抗体水平。
An indirect competitive enzyme-linked immunosorbent assay (ELISA) for detecting Vtg content in the blood of male Carassius auratus was developed with antiserum of lipovitellin resistance as an.
以抗卵黄脂磷蛋白抗血清作为抗体，卵黄蛋白原作为竞争抗原建立了间接竞争法酶联免疫吸附反应(ELISA)方法来检测雄鱼血液中卵黄蛋白原含量。
Objective to develop a indirect ELISA method for the detection of plasma anti-CA iv antibody and to evaluate the relationship between anti-CA iv antibody with pathogenesis of ns, CGN and 2-dn.
目的建立使用间接酶联免疫吸附法(ELISA)检测人血浆抗碳酸酐酶iv抗体的方法，阐明血浆抗碳酸酐酶iv抗体滴度与肾病综征合(ns)、慢性肾小球肾炎(CGN)和2 -型糖尿病肾病(2 - DN)三种肾脏病的联系。
Objective: To detect the expression of ribonuclease inhibitor (RI) in the serum of patients with malignant tumors by indirect ELISA.
目的：探讨间接酶联免疫吸附试验（ELISA）检测核糖核酸酶抑制因子（RI）在恶性肿瘤患者血清中的表达。
Objective: To detect the expression of ribonuclease inhibitor (RI) in the serum of patients with malignant tumors by indirect ELISA.
目的：探讨间接酶联免疫吸附试验（ELISA）检测核糖核酸酶抑制因子（RI）在恶性肿瘤患者血清中的表达。
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