During the process of cooling and thawing, embryos are subjected to a series of cryoinjuries such as cold shock, ice formation, cryoprotectant toxicity and osmotic effect.
超低温保存过程中所造成的冷冻损伤是制约鱼类胚胎超低温保存成功与否的关键，具体表现为渗透压影响、抗冻剂毒性、冰晶损伤等。
During the process of cooling and thawing, embryos are subjected to a series of cryoinjuries such as cold shock, ice formation, cryoprotectant toxicity and osmotic effect.
超低温保存过程中所造成的冷冻损伤是制约鱼类胚胎超低温保存成功与否的关键，具体表现为渗透压影响、抗冻剂毒性、冰晶损伤等。
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