Comparative study on preparation of phosphopeptide from yolk protein and casein hydrolyzed by trypsin was conducted in this paper.
对用胰酶水解酪蛋白和蛋黄蛋白制备磷酸肽进行了比较研究。
While the enzyme hydrolyzed-casein may overestimate the availability of amino acids and endogenous amino acid losses of animal.
酶解酪蛋白法可能高估了内源氨基酸排泄量和氨基酸真消化率。
Methods Casein was hydrolyzed by trysin and chyomtrysin together. The enzymatic hydrolysis was filtrated by ultrafiltrate membrane, and analyzed by SDS-PAGE.
方法将酪蛋白酶解，其酶解产物经超滤膜过滤，用十二烷基磺酸钠——聚丙烯酰铵凝胶电泳（SDS-PAGE）方法分析其酶解情况。
Methods Casein was hydrolyzed by trysin and chyomtrysin together. The enzymatic hydrolysis was filtrated by ultrafiltrate membrane, and analyzed by SDS-PAGE.
方法将酪蛋白酶解，其酶解产物经超滤膜过滤，用十二烷基磺酸钠——聚丙烯酰铵凝胶电泳（SDS-PAGE）方法分析其酶解情况。
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