MTT method is used to evaluate the cytotoxicity of dental materials on human pulp cells.
分别将各组材料的浸渍液与第五代人牙髓细胞共同培养,以MTT法评价材料的细胞毒性。
METHOD: Human pulp cells were primary cultured with tissue block method, enzymolytic method, and tissue block enzymolytic method separately.
方法:分别采用组织块法、酶消化法、组织块酶消化法进行人牙髓细胞原代培养。
AIM: to compare the primary culture methods of human pulp cells, to enhance the culture condition, and give rise of the success rate of cultivation.
前言:目的:比较不同的人牙髓细胞原代培养方法,优化培养条件,提高培养成功率。
Human dental pulp cells were fibroblast-like cells.
人牙髓细胞呈成纤维细胞形。
The result shows that human pulp stroma cells can survive the spaceflight and the space environment can significantly alter the human pulp stroma cells cytoskeleton.
实验结果表明:人牙髓间充质细胞经历空间飞行后可以继续成活,并且细胞骨架发生了明显改变。
CONCLUSIONS: tissue explant method was superior to tissue explant-collagenase digestion method for the culturing of human dental coronal and root pulp cells.
结论:在对根、冠髓细胞的原代培养中,组织块法优于组织块酶解法。
Objective To isolate and identify human dental pulp stem cells from third molars.
目的研究第三恒磨牙来源的人牙髓干细胞的表型和生物学性状。
Objective To study the biological characteristics of cultured human dental pulp stem cells in vitro.
目的研究体外培养的人牙髓干细胞的生物学特性。
It is tentatively concluded that LIM mineralization protein-1 plays a role in the differentiation and mineralization of human dental pulp cells.
结论:首次发现LIM矿化蛋白1与人牙髓细胞的体外矿化过程相关,提示LIM矿化蛋白1可能在牙髓细胞的分化及矿化中发挥一定作用。
PURPOSE: to isolate and culture dental pulp stem cells from human deciduous teeth, then to analyze their biological characteristics and potential to be induced to osteoblasts.
目的:体外分离、培养人乳牙牙髓干细胞,分析其生物学特征及向成骨细胞诱导分化的能力。
PURPOSE: to isolate and culture dental pulp stem cells from human deciduous teeth, then to analyze their biological characteristics and potential to be induced to osteoblasts.
目的:体外分离、培养人乳牙牙髓干细胞,分析其生物学特征及向成骨细胞诱导分化的能力。
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