Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective:To construct plasmid with overexpressed human SGK1 to provide a useful tool for its studies in vitro.
目的：构建人血清及糖皮质激素诱导型蛋白激酶（SGK1）超表达载体质粒，为SGK1的体外研究提供有效的工具。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
结论成功构建了重组人FHIT真核表达质粒。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.
重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
The serum from anemic rats treated with naked plasmid expressing human EPO showed obvious neutralizing activity to inhibit the proliferation of BEF-2 cells.
在大鼠模型中，表达人EPO基因裸质粒组的血清有明显的中和活性，可抑制BEF-2细胞的增殖。
Objective to construct eukaryotic expression plasmid for human insulin-like growth factor 1 (IGF-1).
目的构建人胰岛素样生长因子1 (IGF - 1)的真核细胞表达质粒。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
The screening of human tissue plasminogen activator recombinant plasmid showed that the two methods can recognize the positive colones quickly and correctly.
测序结果表明这两种方法能快速，准确地筛选出阳性克隆。
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
应用推荐