AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。
We construct a marker-free vector of RB gene in order to apply with RB in the tomato late blight breeding and production.
为了使马铃薯抗晚疫病基因RB能够在番茄育种和生产中应用，构建了RB无选择标记载体。
Objective To construct and study the targeted gene therapy system for growth hormone- secreting pituitary tumors.
目的构建并评价垂体生长激素腺瘤靶向性基因治疗系统。
Objective to construct and evaluate the receptor targeted gene therapy system for pituitary adenoma.
目的构建并评价受体靶向性基因导入系统对垂体腺瘤的靶向性治疗作用。
Objective: to construct and identify a lentiviral vector harboring RNAi sequence targeting the human high mobility group A1 (HMGA1) gene.
目的：构建和鉴定人高迁移率族蛋白组a 1 (HMGA1)基因rna干扰慢病毒表达载体。
Objective To construct and express a trichosanthin (TCS) gene mutant and purify the expressed product.
目的构建天花粉蛋白(TCS)突变体基因，并进行表达及纯化。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
目的：构建弓形虫棒状体蛋白（ROP2 ）基因重组质粒并在大肠杆菌中表达，用于筛选弓形虫新的诊断抗原和疫苗分子。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
Objective to construct a polycistronic retroviral vector containing both subunits cDNA of murine IL 12 and NeoR gene and investigate their expressions in murine hepatoma cells.
目的构建含小鼠白细胞介素12双亚基及新霉素磷酸转移酶基因多顺反子逆转录病毒载体，并观察其在小鼠肝癌细胞中的表达。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
目的：构建缺氧诱导表达载体，以介导报告基因在缺氧环境下的特异、高效表达。
Object: to construct the gene array related with immunology gene and bolting the deference related with acute rejection by gene array to express gene.
目的：构建与免疫基因相关的基因芯片，并利用其筛选出与急性排异反应相关的差异表达基因。
To construct a recombinant plasmid containing protein transduction domain (PTD) and brain derived neurotrophic factor (BDNF) fusion gene and express in e.
构建含蛋白转导结构域(PTD)与脑源性神经营养因子(BDNF)融合基因的质粒，并在大肠肝菌中表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the recombinant vector, the expression of human HMGB1 code gene and to study its function.
目的构建人高迁移率族蛋白1（HMGB1）编码基因的表达载体，获得纯化的重组蛋白，研究其生物学功能。
Objective to construct eukaryotic expression vector of human pancreatic duodenal homeobox 1 (PDX-1) gene, and to detect its expression in NIH3T3 cell lines.
目的构建人胰十二指肠同源盒(PD X - 1)基因的真核表达载体，观察其在NIH3T3细胞中的表达。
Objective to construct and express a recombinant adenovirus vector which can regulate expression of human tumor suppressor gene PTEN.
目的体外构建和表达可调控性人抑癌基因pten重组腺病毒。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
AIM: to construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP) -9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.
目的：构建基质金属蛋白酶9靶向的RNA干扰质粒载体，观察其对小鼠巨噬细胞基质金属蛋白酶9基因表达的沉默作用。
Objective To construct the prokaryotic expression vector of the sporozoite surface antigen gene of Eimeria tenella GZ strain and expression in Escherichia coli.
目的构建柔嫩艾美耳球虫子孢子表面抗原原核表达载体，并且在大肠杆菌中表达。
AIM: to construct the recombinant plasmids expressing full-length HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E. coli.
目的：构建丙型肝炎病毒(HCV)全长及3种不同缺失突变的核心蛋白基因的原核表达载体，并在大肠杆菌中表达。
Objective to construct the lentiviral expression vector of HLA-E gene and investigate its significance for further study on tumor immunity.
目的构建表达人类白细胞抗原- E (HLA - E)基因慢病毒载体，探讨慢病毒介导HLA - E基因在肿瘤免疫中的意义。
Objective: to construct a prokaryotic expression vector containing esophageal cancer related gene 2 ECRG2 and observe its expression in Escherichia coli e.
目的：构建食管癌相关基因2ECRG2原核表达质粒，在大肠杆菌e。
Objective To construct a bivalent VP1 gene vaccine against Coxsackie virus B1 and B3 (CVB1 and CVB3) and to test its immunogenicity in mice.
目的构建柯萨奇病毒（CV）B1/B3型二价VP1基因免疫质粒，并探讨其免疫原性。
It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11, a rice variety, by Agrobacterium meditation.
将其与GUS报告基因融合在一起，构建了植物表达载体，并由农杆菌介导法导入水稻品种‘中花11’中。
Objective:To construct a recombinant defective retroviral vector carrying hNT3 gene and a stable virus-producing packaging cell lines , and then to identify them.
构建并鉴定携带人NT - 3基因的逆转录病毒载体以及稳定的包装细胞系。
Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5'-UTR)启动基因表达的活性。
We used random matrix theory (RMT) to remove the noises in lung cancer gene expression data and used the modules approach and the hierarchical clustering approach to construct the gene networks.
利用随机矩阵理论(RMT)方法除去肺癌基因表达数据中的噪声，并将去噪后的数据分别用模块方法和等级聚类方法进行处理。
Amplification VP7 gene from RV G4 serotype, construct a plasmid includes this gene and clone culture.
从轮状病毒G4型中扩增出vp7基因，将该基因构建于质粒并克隆培养。
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