According to the instructions of DNA gel extraction kit, recover purified PCR product.
按照DNA凝胶回收试剂盒的操作说明,回收纯化P CR产物。
AIM To determine the optimal conditions for high DNA extraction efficiency from gels with gel extraction kit.
目的确定提高柱式胶回收试剂盒d NA回收效率的最佳条件。
Ingredient: Deep sea cleanser gene, natural willow extraction, bee gel extraction, corn mint and herbs extraction.
成分:海洋深层洁肤因子、天然柳树皮萃取液、蜂胶萃取液、清爽薄荷和草本植物精华成分。
Genomic DNA of mature leaf in pueraria DC is extracted in SDS method. And the DNA is purified with DNA gel Extraction kit.
采用SDS裂解法提取了葛属植物成熟叶片的基因组dna,并利用凝胶纯化试剂盒进行了纯化。
Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.
对扩增产物进行电泳鉴定,最后将PCR产物经胶回收试剂盒纯化,以备酶切。
Therefore, the SDS-PAGE gel extraction combining with electroosmosis and dialysis recycling are good and easy to purify the inclusion body proteins.
因此,SDS - PAGE切胶纯化后电渗、透析回收是纯化重组包涵体蛋白有效、简便的方法。
Soil microbial total DNA could not be obtained through DNA gel extraction kit after purification with 1% Sepharose containing 2% of PVP (polyvinylpyrrolidone) agarose gel electrophoresis.
含2 %PVP的1 %琼脂糖凝胶电泳纯化,用DNA凝胶回收试剂盒回收后没有得到纯化后的土壤微生物总DNA。
The soybean phosphatidylethanolamine and phosphatidylinositol were prepared by combining methods of solvents extraction and silica gel column chromatography from soybean oil sediment.
采用溶剂萃取法和柱色谱法相结合的工艺,从大豆油脚中制备肌醇磷脂,研究了不同洗脱剂对柱色谱分离脑磷脂和肌醇磷脂效果的影响。
Objective: To optimize the extraction craft of Xiaochuang gel by central composite design-response surface methodology.
目的:采用星点设计-效应面法优选消疮凝胶剂的提取工艺。
Conclusion: The xylanase purification procedure using native polyacrylamide gel electrophoresis combined with homogenization extraction was a novel and convenient method.
结论:活性聚丙烯酰胺凝胶电泳和均质提取法相结合是一种新的分离纯化木聚糖酶的简便、有效方法。
Silica gel column chromatography and solvent extraction were used to separate grease and abradant in a kind of excellent polishing paste for plastics, respectively.
分别采用硅胶柱色谱法和溶剂抽提,分离优质塑料抛光膏中的油脂和磨料部分。
The comparative effects of different treatment methods applied in Aloe gel juice extraction, viz.
本文比较研究了鲜榨、冷榨、热榨和酶解工艺对芦荟凝胶原汁提取效果的影响。
Gel chromatogram was adopted to separate and purify the crude polysaccharide that obtained by ultrasonic-assist extraction.
采用凝胶色谱法对以超声辅助提取法中所得的粗多糖进行分离纯化研究。
Organic phosphorus by acetone extraction, gel column chromatography purification, HP-5 silica capillary column chromatography, flame-ray detector.
有机磷经丙酮提取,凝胶层析柱净化,HP - 5石英毛细管色谱柱分离,火焰光检测器检测。
Organic chlorine using ethyl acetate - cyclohexane and gel purification column extraction purification, quartz capillary gas chromatography (ECD allocation) detection.
有机氯类用乙酸乙酯—环己烷和凝胶净化柱提取净化,石英毛细管气相色谱(配ecd检测器)检测。
The products obtained were purified by extraction with copper sulfate solution, filtration through silica gel, recrystallization and vacuum sublimation.
粗产物经硫酸铜溶液萃取、硅胶过滤、重结晶、真空升华进行分离提纯得到纯产品。
This review deals with the progress in the studies of silica gel bound crown for extraction and separation of metal ions. The preparation, application and instrument are described in detail.
综述了冠醚改性硅胶萃取分离金属离子的研究进展,对其制备、应用等也进行了详细的介绍。
Methods Silica gel superfine process was conducted by combining solvent extraction and separation with macroporous absorbent resin.
方法首先用溶剂萃取结合大孔吸附树脂分离,然后用硅胶柱精制提取。
Conclusion: the application of "four-step extraction" method enhances the peptide recovery during in-gel digestion, and increases identification of the protein.
结论:应用“四步提取法”使得酶解肽段提取率和蛋白质的有效鉴定率明显提高。
Method: Compounds were separated by solvent extraction and column chromatography with silica gel and structures were elucidated by chemical evidence and spectral analysis.
方法:溶剂提取和硅胶柱色谱法进行分离,理化性质和光谱数据鉴定其结构。
Bio-transforming the canrenone with the enzyme system of Aspergillus ochraceus SIPI-4411; By solvent extraction and silica gel column chromatography, the compound SIPI-CANR was obtained.
应用赭曲霉SIPI-4411的羟化酶系统对化合物坎利酮进行生物转化,通过溶剂萃取、硅胶柱层析,获得转化产物SIPI-CANR化合物的纯品。
Objective To explore an optimal cartilage protein extraction approach that can guarantee both the image quality and the result fidelity of the two-dimensional gel electrophoresis (2-DE) technique.
目的探索可兼顾双向电泳凝胶图像质量和结果保真性的软骨蛋白提取方案。
Objective:To investigate the changes in vitreous gel structure after phacoemulsification lens extraction in rabbit eyes.
目的:探讨超声乳化晶体摘除术后玻璃体凝胶结构的改变。
The oxygen containing components are obtained from Gudong Crude oil of Shengli by solvent extraction and separated into acidic and other components chromatographically on silica gel column.
用溶剂萃取法从胜利孤东原油中萃取、浓缩出含有机酸的活性组分,用柱色谱分离成窄馏分并进行了波谱鉴定。
Ultrahigh molecular weight polyethylene fibers(UHMWPE) of different gel concentration were prepared using gel spinning process followed by solvent extraction, drying and drawing.
采用凝胶纺丝工艺制备了具有不同质量分数的超高分子质量聚乙烯凝胶纤维,并对其进行了萃取干燥和后拉伸等处理。
Protein component of sika deer antler base (PSAB) was extracted by means of salty extraction, boiling and gel filteration.
采用盐提、热变性处理及凝胶过滤法从鹿花盘中提取鹿花盘蛋白质(PSAB)。
The extraction rate of carrageen was increased, while some shortcomings such as that the gel of the carrageen being prone to bleeding water and shrinking were improved under the above method.
提高了卡拉胶的提取率,完全改善了卡拉胶凝胶易收缩析水等缺陷。
The ethyl acetate and n-butanol extraction were chromatographed on silica gel column, polyamide column, sephadex LH-20 column and preparing TLC, respectively.
采用硅胶、聚酰胺、葡聚糖凝胶LH-20柱层析法和制备薄层层析法对乙酸乙酯和正丁醇提取物进行了分离、纯化。
The limonin sample is got by extraction with chloroform and is separated by a thin-layer chromatography of silica-gel G, and then is determined by colorimetric metho…
柠檬苦素采用氯仿萃取取样,并经硅胶G薄层分离后以对——二甲氨基苯甲醛作显色剂比色测定。
The limonin sample is got by extraction with chloroform and is separated by a thin-layer chromatography of silica-gel G, and then is determined by colorimetric metho…
柠檬苦素采用氯仿萃取取样,并经硅胶G薄层分离后以对——二甲氨基苯甲醛作显色剂比色测定。
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