Objective to explore the optimal condition of direct differentiation into dopaminergic neurons of embryonic stem cells in serum-free and feeder layer cell free medium.
目的寻求无血清、无饲养层细胞存在的情况下，胚胎干细胞向多巴胺能神经元定向诱导分化的最佳条件。
Selection of the feeder layer, medium, and supplemental cytokines and their roles in establishing and maintaining human ES and EG cell lines at present are illustrated in detail systematically.
详细系统地说明了迄今为止建立人胚胎干细胞系所需要的饲养层类型、培养基组成、添加细胞因子种类及其作用；
Rat tracheal epithelial cell (RTE) grows well in vitro under the support of 3t3 feeder cell layer.
原代rte细胞在3T3滋养细胞支持下，在体外培养条件下可生长。
Without feeder layer, human embryonic stem cell differentiation was observed in vitro.
撤除饲养层，观察人胚胎干细胞体外分化情况。
Without feeder layer, human embryonic stem cell differentiation was observed in vitro.
撤除饲养层，观察人胚胎干细胞体外分化情况。
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