Then, antigen was prepared by conjugation of hapten with carrier protein by carbodiimide (EDPC) method and was identified by UV spectroscopy and SDS-PAGE.
然后通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原，采用紫外扫描及SDS - PAGE鉴定。
Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS. At the presence of EDPC, T 2HS conjugated with BSA, then BSA T 2HS was prepared.
方法T-2毒素与琥珀酸酐在吡啶参与下于蒸汽浴中反应，生成T- 2 - 半琥珀酸酐后，再与小牛血清白蛋白经碳二亚胺偶联结合生成T- 2毒素抗原。
Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS. At the presence of EDPC, T 2HS conjugated with BSA, then BSA T 2HS was prepared.
方法T-2毒素与琥珀酸酐在吡啶参与下于蒸汽浴中反应，生成T- 2 - 半琥珀酸酐后，再与小牛血清白蛋白经碳二亚胺偶联结合生成T- 2毒素抗原。
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