Objective to investigate the correlation between phenotypic confirmatory by double disc test and genotype of CTX-M-enzymes detected by multiplex polymerase chain reaction (PCR).
目的了解采用双纸片协同法进行CTX - M酶的表型检测与多重pcr法检测基因型的相关性。
The duplex PCR and double digestion technique were established to detect polymorphisms of ESR and RYR1 simultaneously.
探讨和初步建立了利用双重pcr和双酶切技术同时检测esr、RYR1两个基因的变异的方法。
A double - round reverse transcriptase - coupled polymerase chain reaction (RT - PCR) was applied to detect the CYP1A1 transcript.
双轮回‘聚合酶链式反应’(PCR)和‘反向转录酶-聚合酶链式反应’(RT - PCR)被用来检测基因CYP1A1的转录水平。
It was demonstrated that the negative results in direct FA assay and the double antibody sandwich ELISA were found, but the result in RT-PCR was positive.
结果死亡病人脑组织的直接FA试验和双抗体夹心elisa检测为阴性，但RT - P CR检测结果为阳性。
It was demonstrated that the negative results in direct FA assay and the double antibody sandwich ELISA were found, but the result in RT-PCR was positive.
结果死亡病人脑组织的直接FA试验和双抗体夹心elisa检测为阴性，但RT - P CR检测结果为阳性。
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