The partial digest (PDP) and the simple partial digest (SPDP) methods are the popular methods of DNA restriction mapping reform.
DNA限制性图谱绘制常用方法有部分消化法（PDP）和简化的部分消化方法（SPDP）。
Compaired with the strains of Campylobacter coli and loridis from Belgium, the patterns of DNA restriction fragements was different.
与从比利时引进的结肠弯曲菌及海鸥弯曲菌比较，带型完全不同；
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means for DNA restriction enzyme pattern analysis.
结果表明，CE的分离效能明显高于age，是研究d NA限制性内切酶谱的更有效的检测手段。
Secondly, we have designed cross network graph so as to construct and analyse the DNA restriction physical maps. Examples ars given to show the advantages of the approach.
提出一种DNA物理图谱的交叉位置网络图（简称交叉位置图），并通过实例说明它在构建物理图谱和分析酶切实验中的应用意义。
Bacteria have defences against viruses in the form of chemicals called restriction enzymes, which chop up foreign DNA.
细菌有一种对病毒的防御机制：一种叫做限制性内切酶的化学物质可以切断外源dna。
The method USES fluorescence microscopy to image individual DNA molecules that have been divided into orderly fragments by so-called restriction enzymes.
该方法应用荧光显微术使单个DNA分子成像，这些单个的DNA分子已经被分成有序的片断，因而被称为限制性内切酶的识别序列。
Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
首先用限制性内切酶将大的DNA分子切断，产生出特殊的片段。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作，无需使用限制性内切酶和连接酶。
The applications of karyotyping and restriction fragment length polymorphism (RFLP) analyses, specific DNA probes and PCR method to the diagnosis of Candida albicans infections are reviewed.
综述了白色念珠菌感染的分子生物学诊断研究进展，包括核型分析、限制性片段长度多态性分析、特异的DNA探针及PCR等方法。
When genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the DNA was methylated or not.
结果显示不论DNA甲基化与否，所有使用受体细胞限制性内切酶的基因组移植都获得成功。
Eight restriction endonucleases were used to investigate mitochondrial DNA polymorphisms of takin, cattle, sheep, buffalo and goat in Bovidae.
用8种限制酶分析了种牛科动物，羚牛、黄牛、水牛、绵羊和山羊的线粒体dna的多态性。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（ RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme.
锌指核酸酶是一种人工制做限制性内切酶，通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。
Restriction enzyme: Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.
限制性内切：由细菌产生的一种蛋白质，能在特定的地方切断去氧核糖核酸分子。收藏。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
DNA modification with restriction-modification system is a component of microbial immune system, and DNA modification without restriction system influences phenotypes by gene regulation.
由限制-修饰系统调控的DNA修饰参与微生物的免疫防御系统，无限制-修饰系统调控的DNA修饰通过调控基因表达影响表型。
Methods Large restriction fragment (LRF) pattern analysis of genomic DNA by using pulsed field gel electrophoresis was performed and differences of patterns were compared.
方法采用核酸脉冲电泳场分析技术，分析5 3株临床分离株酶切电泳图谱，并比较图谱的差异。
Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
应用推荐