• Then they searched for DNA amplification enzymes that could operate at that thermal cycling speed.

    然后他们再寻找能够在这种环境正常工作的放大酶。

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  • The DNA amplification technique is used in genome sequencing, forensics and the diagnosis of various diseases.

    这种DNA的放大技术被用于多种疾病的检验,诊断和基因组序列的测定。

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  • An integrated microstructure PCR-DNA amplification biochip based on MEMS technology was fabricated, which includes heater, temperature sensor, reaction chamber and inlet channels.

    基于MEMS技术研制了微结构的集成PCR-DNA分子扩增生物芯片,芯片集成了加热子、温度传感器、反应室、进样通道等;

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  • The reactions are typically carried out on a regulated heating block, or PCR machine, and consist of 30-35 cycles of repeated amplification of all the DNA present.

    这一反应通常是在一可调控的温箱中或PCR仪中进行,对所有的DNA进行30到35个循环扩增。

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  • Sequencing technology has come a long way since the Human Genome Project, but it still requires DNA to be copied millions of times, a step called amplification, and labelled with fluorescent tags.

    测序技术在人类基因组计划后有了长足的进展,但是它仍然要求DNA经过一项称为“放大”的步骤,被复制几百万次,然后再用荧光标记。

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  • With nanopores, however, virtually no chemical pre-processing is required and no amplification. Instead a single strand of DNA can be read, one base pair at a time, and without the need for labelling.

    但是如果用纳米孔,实质上就不需要化学与处理和“放大”,取而代之的是,可以一次一个碱基对地读出单条dna链,也不用标记。

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  • Genetic damage with DNA alterations leads to point mutations of genes, translocations of genetic material between chromosomes, and gene reduplication with amplification.

    伴随DNA改变的基因损伤会导致基因的点突变、染色体间基因移位和扩增性基因复制。

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  • DNA Extracted from Nanyang yellow cattles hair follicle and blood was used to RAPD amplification.

    从南阳黄牛被毛毛囊细胞和血液中分别提取DNA用于RAPD扩增。

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  • OBJECTIVE To establish the random amplification of polymorphic DNA (RAPD) typing technology for Proteus strains and apply it in the epidemiology investigation.

    目的建立变形菌随机扩增dna多态性分型技术,应用于临床菌株流行病学调查。

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  • Apparatus for immobilized DNA library preparation, apparatus for gene amplification, method for temperature control and method for comparing genes systematically.

    用于制备固定化dNA文库的装置,基因扩增的装置,温度控制方法以及系统比较基因的方法。

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  • Therefore, the paper suggests PCR amplification DNA is a highly sensitive and specific method for diagnosing tuberculous exudative pleurisy early and rapidly.

    提示PCR扩增DNA技术对结核性渗出性胸膜炎是高度敏感和特异的早期、快速诊断方法。

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  • CDK4 amplification could be correlated with HBV DNA integration and the progression of HCC.

    CDK4的扩增可能与HBV -DNA的整合以及肝癌的进展有关。

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  • Random amplification of polymorphic DNA(RAPD)assay was used to study the finger-printing of 26strains from 16specie s.

    以随机扩增DNA多态性法对16种支孢26株菌的DNA指纹图谱作了分析。

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  • A method for quickly preparing PCR amplification of dinoflagellate total DNA template was reported.

    报道了一种快速制备P CR扩增甲藻细胞模板dna的方法。

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  • Validity oft his "non-DNA" principle was evaluated by amplification of globin gene from fresh tonsil tissues and plasmid gene on condition that gloves were contaminated by plasmid.

    然后通过扩增新鲜扁桃体组织中珠蛋白基因及模拟手套被质粒污染的情况下扩增质粒基因,验证该无DNA操作过程对避免污染的有效性。

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  • Rare amount of DNA sample often needs repeated amplification in order to remain enough amount of DNA template for various analysis.

    基因操作者经常需要把数量稀少的DNA样品进行反复扩增以供进一步研究之用。

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  • A method named selective amplification DNA fragments (SADF) using single restrictive enzyme was established and optimized in barley.

    建立和优化了大麦dna单限制性酶切选择性扩增多态性技术体系(SADF)。

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  • Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.

    方法:PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。

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  • The method to extract genome DNA from old skeletal specimens of cetaceans was improved and the PCR amplification reaction was optimized.

    改进了从鲸类陈旧骨骼标本中进行基因组dna提取和PCR扩增的方法。

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  • CONCLUSIONS HBV DNA extracted from clinical samples were directly detected using PNA biosensor and PCR amplification was successfully bypassed.

    结论利用肽核酸生物传感器成功地绕过了PCR扩增而直接检测出了临床标本中的HBV基因组dna。

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  • Objective to research on the possibility and the efficiency of the enzyme biological signal amplification system applied to DNA piezoelectric biosensor.

    目的研究“酶生物信号放大系统”用于放大dna压电传感器检测信号的可行性和有效性。

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  • Objective: To introduce a simple rapid exactive modified PCR for detection CMV DNA in amniotic fluid strand displacement amplification for prenatal diagnosis of congenital HCMV infections of fetus.

    目的:介绍一种简便快速准确检测羊水中CMV-DNA的改良PCR-链置换式扩增用于诊断胎儿先天感染CMV。

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  • The modified SDS method which was used to extract DNA got good results. During RAPD , RNA in the DNA template had no effects on the amplification results.

    利用改良的SDS法提取DNA效果很好,RAPD反应时,DNA模板中RNA对扩增结果无影响,SDS、氯仿、异丙醇等小分子物质对扩增结果有干扰作用。

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  • Result in the PCR amplification sensitivity testing revealed that 0.021 egg DNA template would be enough to amplify this specific positive band.

    其PCR扩增敏感性检测结果表明,0.021个虫卵dna模板即可扩增出该特异性阳性条带。

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  • Results: DNA extraction and polymerase chain reaction amplification was successful in all cases.

    结果:所有病例都成功地进行了DNA提取和多聚酶链反应扩增。

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  • Objective To study the fetal DNA in maternal plasma using multiplex PCR amplification of the short tandem repent (STR) systems.

    目的采用短串联重复序列(STR)多态位点的复合扩增方法,研究孕妇血浆中胎儿DNA基因型。

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  • Objective To study the fetal DNA in maternal plasma using multiplex PCR amplification of the short tandem repent (STR) systems.

    目的采用短串联重复序列(STR)多态位点的复合扩增方法,研究孕妇血浆中胎儿DNA基因型。

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