Conclusions There is a trend that can be recognized in the process of designing primer. Namely, the forepart is far more than the anaphase in the ratio of success to design primer.
结论从引物设计的过程中可以看到一种趋势-后期引物设计的成功率远远高于早期。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degenerate PCR.
成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。
The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used.
引物设计是RAMP和REMAP标记分析的关键，二者都使用锚定的微卫星序列引物。
Primer design is the key to our success, the primer sequences have designed specifically according to the reference.
引物的设计很关键，特异的引物序列设计参照文献。
Integrated graphic sequence feature viewer to assist primer design.
整合图形化序列特征，进一步协助您设计引物。
Here, a detailed protocol for bisulphite conversion, primer design, and optimization of PCR conditions is given.
这里，给出了亚硫酸氢盐转换，引物设计和PCR条件优化的一个详细的实验方案。
This review summarized the recently research process of Sq RT-PCR. It including extraction of total RNA, primer design, definition of cycle count, selection of inner reference and so on.
该文就半定量RT-PCR法的检测步骤及技术的关键因素（总RNA提取、引物设计、循环数的确定和内参的选择等）进行综述。
SeqState - primer design and sequence statistics for phylogenetic DNA data sets.
进化DNA数据的引物设计序列统计软件。
Our main concern while using this method involves sensitivity, fidelity and the ease of primer design.
在应用这一方法时，我们最关心以下三个方面：敏感性，忠实性和引物设计的方便。
This paper introduced the source of exhaust gas from cathodic electrodeposition primer in baking, and gave the design principle of disposal equipments.
介绍了阴极电泳漆烘干过程中废气的来源，提出了废气处理设备的设计原则。
The authors may be contacted regarding other requirements for primer design.
关于引物设计的其他需求，可以联系作者。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
In addition, common questions encountered in primer design were discussed and the corresponding measures were put forward.
同时，对引物设计过程中常见问题进行了讨论并给出了解决办法。
Fig. 1. Design strategies for STV11 marker. Primer binding sites are shown in red, sites with different sequence are shown in blue.
图1抗性基因STV11引物设计策略红色表示引物结合位点，蓝色表示序列差异位点。
Fig. 1. Design strategies for STV11 marker. Primer binding sites are shown in red, sites with different sequence are shown in blue.
图1抗性基因STV11引物设计策略红色表示引物结合位点，蓝色表示序列差异位点。
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