Objective to reconstruct rabbit corneal epithelium in vitro with a free-feeding layer culture system and autograft to treat limbal stem cells deficiency.
目的采用无饲养层细胞培养体系构建家兔角膜上皮组织，自体移植后观察其对角膜缘干细胞缺失的治疗效果。
Conclusion the reconstructed rabbit corneal epithelium hi vitro with a free-feeding layer culture system could be autografted to repair total corneal limbal stem cells deficiency.
结论采用无饲养层细胞培养体系构建自体角膜上皮组织，移植后可以成功修复角膜缘干细胞缺失的角膜上皮。
Then, a laser is used to reshape the lower layer of corneal tissue.
然后，用激光修整下层角膜组织的形状。
Conclusion the migration of corneal epithelial stem cells in the human fetuses was from the whole layers to basal layer and confined to limbus region finally, and their ultrastructure was immature.
结论胎儿角膜上皮干细胞呈现由全层向基底层，再向缘部逐渐迁移的分布规律，且超微结构较幼稚。
Conclusion the migration of corneal epithelial stem cells in the human fetuses was from the whole layers to basal layer and confined to limbus region finally, and their ultrastructure was immature.
结论胎儿角膜上皮干细胞呈现由全层向基底层，再向缘部逐渐迁移的分布规律，且超微结构较幼稚。
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