After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color.

一会儿之后孵化，洗去移除所有绑定的基体的解酶，补充说，这是通过把作用于酶发生颜色。

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After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color.

一会儿之后孵化，洗去移除所有绑定的基体的解酶，补充说，这是通过把作用于酶发生颜色。

youdao