Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers, a novel megaprimer method was performed in one tube with two steps and 34 cycles.
采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。
Gain many clone of gene fragment on differential expression, choice 3 of them for sequencing, and blast the sequence in Genebank.
获得了很多差异片段的克隆，并从其中选择3个进行测序。
Results four positive clones of differential screening were picked out for sequencing. Homology analysis indicated that all of the four clone sequences were the same as that of insulin-I gene.
结果挑选了4个差异筛选的阳性克隆进行测序，序列同源性分析表明它们均与胰岛素- i基因序列高度一致。
Results four positive clones of differential screening were picked out for sequencing. Homology analysis indicated that all of the four clone sequences were the same as that of insulin-I gene.
结果挑选了4个差异筛选的阳性克隆进行测序，序列同源性分析表明它们均与胰岛素- i基因序列高度一致。
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