Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.
结论：可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
The plasmid was transformed to E. coli BL21(DE3) by screening and identifying to obtain the high expressing positive clone.
再将此等质粒转化至大肠杆菌，经过筛选鉴定获得高表达阳性克隆。
Results four positive clones of differential screening were picked out for sequencing. Homology analysis indicated that all of the four clone sequences were the same as that of insulin-I gene.
结果挑选了4个差异筛选的阳性克隆进行测序，序列同源性分析表明它们均与胰岛素- i基因序列高度一致。
Results four positive clones of differential screening were picked out for sequencing. Homology analysis indicated that all of the four clone sequences were the same as that of insulin-I gene.
结果挑选了4个差异筛选的阳性克隆进行测序，序列同源性分析表明它们均与胰岛素- i基因序列高度一致。
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