Methods Chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro. Chondrocyte culture media was collected.
方法用酶消化、分离鸡胚关节软骨细胞,体外单层培养,收集细胞培养液。
Objective To investigate the feasibility of amplifying big animal chondrocytes in vitro by using rotary cell culture system (RCCS).
目的探讨用旋转细胞培养系统(RCCS)进行大动物软骨细胞扩增的可行性。
Conclusion Using this method to isolate and culture rabbit's articular chondrocytes is feasible.
结论采用本方法培养兔软骨细胞是可行的。
Bone marrow stromal stem cells isolated from bone marrow are the special mesenchymal stem cells, which can be differentiated in culture into osteoblasts, chondrocytes, adipocytes, and even myoblasts.
骨髓基质干细胞是一种由骨髓中分离获得的具有多种分化潜能的间质干细胞。在体外培养条件下,它可以分化为成骨细胞、软骨细胞、脂肪细胞、甚至于成肌细胞。
AIM: to observe the induction and differentiation of marrow mesenchymal stem cells (MSCs) into chondrocytes under dynamic microgravity and three dimensions (3d), and compare it with static culture.
目的:观察微重力条件下动态三维诱导骨髓间充质干细胞向软骨细胞的分化,并与静态培养作比较。
The methods of isolation and culture sika deer antler chondrocytes were established.
建立梅花鹿鹿茸软骨细胞的分离培养方法,培养鹿茸软骨细胞。
Results Chondrocytes without scaffold formed cartilage which obtained some hyaline like histological characteristics by culture in centrifuge tube.
结果软骨细胞无支架培养形成软骨,具有一定的骺软骨组织学特征。
Methods Coated PLA, PGA, PLGA with poly-l-lysine, free chondrocytes isolated from rabbit articular cartilage were seeded onto three kinds of scaffolds after expansion by in vitro culture.
方法多聚赖氨酸包埋PLA,PGA,PLGA三维细胞支架。分离培养兔关节软骨细胞,体外扩增后种植到三种支架中。
Abstract: Objective to discussion the feasibility of mixed culture muscle-derived stem cells and chondrocytes to chondrogenesis in vitro.
摘要:目的探讨肌源性干细胞与软骨细胞混和培养体外成软骨的可行性。
Abstract: Objective to discussion the feasibility of mixed culture muscle-derived stem cells and chondrocytes to chondrogenesis in vitro.
摘要:目的探讨肌源性干细胞与软骨细胞混和培养体外成软骨的可行性。
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