Feed streams consisted of CHO cells spiked with the viruses.
超滤中,进料流中含有这些病毒的CHO细胞成份。
Objective: To study the expression of murine stem cell factor in CHO cells.
目的:克隆造血干细胞因子并在CHO细胞中表达。
Objective To improve the immunogenicity of recombinant HBsAg expressed in CHO cells.
目的提高CHO细胞表达的重组乙型肝炎病毒表面抗原的免疫原性。
Objective To evaluate the growth of CHO cells in serum-free and serum-containing media.
目的对无血清培养基与含血清培养基培养cho细胞进行比较。
Conclusion At present, the serum-containing medium for culture of CHO cells can not be completely replaced by serum-free serum.
结论目前无血清培养基尚不能完全取代含血清培养基用于培养CHO细胞。
Recombinant CHO cells showed significant base-resistance because a great deal of acidic metabolites were produced in the course of culture.
CHO工程细胞在培养中产生大量酸性代谢产物,因而耐碱性强。
CONCLUSION: MTX induced-increase of recombinant antibody production in CHO cells can be increased by weakening the expression of DHFR gene.
结论:弱化表达载体中DHFR的表达,可提高MTX对工程抗体表达的增加效果。
METHODS: Using CHO cells which high express human CD59 as target cells, the phage 12 peptide library was screened for 5 rounds by competitive binding test.
方法:以高表达人CD59的中国仓鼠卵巢细胞(CHO)为靶细胞,采用竞争结合试验,对噬菌体随机12肽库进行5轮亲和筛选。
Objective: Through suspension adaptation, to endow the Chinese hamster ovary cells (CHO cells) with suspension growth characteristics and rapid growth ability.
目的:通过悬浮适应,使中国仓鼠卵巢细胞(CHO细胞)获得悬浮生长的特性,并可在悬浮培养条件下较快地生长。
Objective:To establish a stable CHO cell line expressing GLUT4-EGFP, and to lay a foundation for investigating the translocation mechanisms of GLUT4 in CHO cells.
目的:建立稳定表达EGFP标记的葡萄糖转运蛋白4的CHO细胞系,为研究GLUT4在CHO细胞中的转运调节机制奠定基础。
Methods:Culture CHO cells in serum-free media and then compare the maintainance time, morphological changes and hemagglutination titers of the cells in culture supernatant.
方法:应用无血清培养基采用小方瓶培养CHO细胞,观察细胞维持时间、培养过程的形态变化。
Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.
目的构建人黑色素浓集激素1型受体(MCHR1)真核表达载体,转染CHO细胞,建立稳定转染的CHO细胞系。
Objective: to stably transfect imidazoline-1 receptor (I 1r) in Chinese hamster ovary (CHO) cells.
目的:在中国仓鼠卵巢(CHO)细胞上稳定转染咪唑啉1受体。
Objective To compare the culture effects of CHO-C28 cells by domestic and imported DMEM.
目的比较国产与进口DMEM培养基培养cho - C28细胞的效果。
The ultrastructure of prematurely condensed chromosomes (PCC) in BK cells and the CHO metaphase chromosomes (the PCC inducer) were studied with cell fusion technique and SEM.
应用细胞融合技术和扫描电镜研究了BK(牛肾)细胞早熟凝集染色体(PCC)和诱导PCC的CHO中期染色体的超微结构。
Conclusion: MTX selection should be timely used to overcome the instability of the expression of anti HBsAg in CHO HB2 cells.
结论:应及时用MTX对细胞株进行再加压筛选以及保存,以保持细胞稳定表达抗体的能力;
AIM: TO obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR-cells.
实现其在CHO - DHFR -细胞中的高效表达,获取生物活性较高的重组蛋白。
AIM: TO obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR-cells.
实现其在CHO - DHFR -细胞中的高效表达,获取生物活性较高的重组蛋白。
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