The result shows that routine phenol-chloroform method was adapt to extract a mass of DNA.
结果表明,常规酚—氯仿抽提法适合从马血中大量提取DNA。
Methods: 85 clinical strains of S. aureus were extracted by phenol chloroform method and the RAPD typing was also performed.
方法:对临床分离的85株金黄色葡萄球菌,用酚氯仿法提取DNA后进行RAPD分型。
METHODS DNA from 15 clinical strains of S. aureus were extracted by phenol-chloroform method and used in the RAPD assay, phage typing were also performed.
方法对临床分离的15株金黄色葡萄球菌,酚氯仿法提取其DNA后行RAPD分型,同时进行噬菌体分型。
Objective To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.
目的建立运用酚-氯仿法结合磁珠法从蝇蛆嗉囊内提取人类DNA的方法,从而提高STR分型检验的灵敏度。
The chloroform solution of 4-Amino-antipyrine was used to measure the phenol in water by extraction and photometer. The method and the precision were discussed in this paper.
本文通过实验的方法,用三氯甲烷处理4- 氨基安替比林溶液,解决了用4-氨基安替比林测定挥发酚实验空白值高的问题,并确定了三氯甲烷最佳使用量。
Using 70% methanol, the Polygonum multiforum extract was concentrated by ultrasonic method . Then concentrated extract was leached with chloroform and separated into free and bound anthraquinones.
何首乌用70%甲醇超声提取浓缩后,用氯仿萃取分离出游离和结合型蒽醌,得到定量用试液。
An improved method of synthesizing trimethyl orthoformate through azeotropic distillation by taking sodium methoxide in methanol and chloroform as raw materials has been presented by the author.
介绍了以甲醇钠甲醇溶液与氯仿为原料,采用共沸蒸馏法制备原甲酸三甲酯的改进工艺。
Furthermore, we found the best HPLC spectrum of Fritillaria Jilin was soxhlet method with the solvent of chloroform and methanol (4:1), the others were no better than it.
经氯仿:甲醇(4:1)混合溶剂索式提取的贝母药材HPLC图谱效果最好,其它次之。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA,比较荧光定量pcr测定的重复性和测定值的差异。
The results suggest that this method, though simple, can be used to prepar template for PCR from various cells and tissues without extracting DNA by SDS-phenol-chloroform.
揭示该方法能从各种细胞和组织中制备pcr的模板,而无需用SDS -苯酚-氯仿抽提dna。
The chloroform fumigation-incubation method was used to measure the biomass-C and-N of soils with different fertility levels.
应用氯仿熏蒸培养法测定不同肥力水平的土壤生物量-C和-N。
According to the volatility of chloroform, the method for determining the chloroform in drinking water was established by gas chromatography with head-space sampling.
利用氯仿的易挥发性,建立了顶空进样气相色谱法测定饮用水中氯仿的分析方法。
A chemical labyrinthectomy caused by chloroform and the method of quantitative analysis of the movement of guinea pig's eyes were also discussed in this paper.
初步探讨了化学性(氯仿)迷路破坏的动物模型及豚鼠眼运动的定量观察方法。
DNA was extracted from stools by phenol chloroform extracting method.
通过改良酚氯仿抽提法提取粪便dna。
The limonin sample is got by extraction with chloroform and is separated by a thin-layer chromatography of silica-gel g, and then is determined by colorimetric method where pdi...
柠檬苦素采用氯仿萃取取样,并经硅胶g薄层分离后以对——二甲氨基苯甲醛作显色剂比色测定。
Methods: To compare the method of the extract of RNA from goldfish using laminar analysis column with that of using one-step guanidinium isothiocyanate-alcohol phenyl-chloroform.
方法:应用本层析柱方法从金鱼组织所提取RNA和采用异硫氰酸胍-酚-氯仿一步法提取RNA的比较。
A simple capillary GC method for the determination of residual ethanol, dichloromethane, chloroform, tetrahydrofuran, 1, 4 dioxane, pyridine and toluene in tropisetron citrate was established.
采用毛细管气相色谱法,以外标法测定枸橼酸托烷司琼中乙醇、二氯甲烷、氯仿、四氢呋喃、二烷、吡啶和甲苯的含量,用串联的HP -1 0。
Method Methanol and mixed solvent of chloroform and ethyl acetate extracts from Cordyceps formosana were analyzed with HPLC-DAD-HRMS.
方法用高分辨液质联用分析法,对其甲醇提取物及二氯甲烷和乙酸乙酯混溶剂提取物进行了成分分析。
Method Methanol and mixed solvent of chloroform and ethyl acetate extracts from Cordyceps formosana were analyzed with HPLC-DAD-HRMS.
方法用高分辨液质联用分析法,对其甲醇提取物及二氯甲烷和乙酸乙酯混溶剂提取物进行了成分分析。
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