Chromosomal instability increased as the cell passage increased.
随着传代次数增加，染色体不稳定性更明显。
Methods PC12 cells and BV2 cells, instead of neurons and microglia separately, were co-cultivated in transfer screen by cell passage method.
方法采用细胞株传代法培养PC12细胞及BV2细胞，分别替代神经元细胞和小胶质细胞；
An ion channel is a molecule that allows the passage of electrically charged atoms of sodium, potassium, calcium and so on through the outer membrane of a cell.
离子通道是一种分子，它允许钠、钾、钙等离子经由它通过细胞外膜。
Influences of embryo quality and type differentiation stayer media passage and digestion fluid on embryonic cell separation and cloning were discussed respectively.
本文就胚胎质量和胚胎类型分化抑制物培养基传代时间和消化液等方面对胚胎干细胞分离克隆的影响进行了讨论。
In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.
另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。
The transient properties(mean first passage time T)of the tumor cell growth system driven by color Gaussian noise are investigated.
研究了受色高斯噪声驱动的肿瘤细胞增长系统的瞬态态性质（平均首通时间T）。
Objective to investigate the biological features and immunophenotypes of human glioblastoma cell line SHG44 after long term passage.
目的探讨人脑胶质母细胞瘤细胞系SHG44长期传代后的某些生物学特性和免疫表型特征。
The primary isolated strain was used to infect human transformed T-cell lines MT4, and a stable passage strain 29A was gained.
方法：用从该患者外周血单核细胞中分离的病毒株感染MT4细胞，获得可在该细胞上稳定传代的病毒株29A。
Infection rates, titer, and OB yield declined with increasing serial passages of MsNPV in the cell line, they dropped off rapidly after the 7th or 8th passage.
病毒连续传代至第7代或第8代以后，其感染率、病毒滴度及多角体产量均有显著下降。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
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