Result of identification of PACCMV-BMP2 shuttle plasmid.
BMP2穿梭质粒的鉴定结果。
The changes of BMP2 and osteopontin were detected by immunohistochemistry and Western blotting.
骨形态发生蛋白2与骨桥蛋白检测：采用免疫组织化学染色与蛋白印迹方法。
Conclusion: BMP2 gene may be transfered and expressed in skeletal muscle cells by direct injection.
结论：直接注射BMP2基因可使其导入骨骼肌细胞内并表达。
Objective To clone the human recombined bone morphogenetic protein 2 (BMP2) gene in Escherichia coli.
目的：在大肠杆菌中克隆人骨形成蛋白2基因。
Results: Inflammatory reaction was observed in the gene-injected area and some skeletal muscle cells demonstrated BMP2 positive signals.
结果：注射部位局部有炎症反应，部分骨骼肌细胞内可见BMP2的阳性信号。
Conclusion: Lower value and the lag in genic expression of BMP2 in the central zone during the early stage may be an important reason of nonunion occurring in rat femur.
结论：大鼠股骨骨不连中心区在修复早期BMP2基因的低表达及表达时间滞后可能是其骨不连发生的原因之一。
Conclusion: Lower value and the lag in genic expression of BMP2 in the central zone during the early stage may be an important reason of nonunion occurring in rat femur.
结论：大鼠股骨骨不连中心区在修复早期BMP2基因的低表达及表达时间滞后可能是其骨不连发生的原因之一。
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