Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体由合成化肽段免疫动物，该合成肽段与YAP人源蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。抗体用蛋白a和多肽亲和色谱法纯化。
Antibodies are purified by protein A and peptide affinity chramotography.
抗体由蛋白A和肽段亲和层析纯化得到。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CDK5. Antibodies are purified by protein A and peptide affinity chromatography.
该多克隆抗体由合成的人类CDK5氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体由蛋白a和肽段亲和层析技术纯化。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
The recombinant DHFR protein containing his-tag was purified by using TALON metal affinity resin under nondenaturing conditions.
在非变性条件下，用TALON金属亲和层析树脂纯化含组氨酸标记的重组DHFR。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKM2. Antibodies are purified by protein A and peptide affinity chromatography.
该多克隆抗体用与人类PKM2蛋白氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA. Antibodies are purified by peptide affinity chromatography.
该多克隆抗体用与人类LDHA氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白亲和层析纯化而得。
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein a and peptide affinity chromatography.
该抗体通过使用合成肽段免疫动物而获得，并经蛋白a和肽亲和层析纯化。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography (MCAC).
研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human APC. Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于人源APC蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I. antibodies are purified by peptide affinity chromatography.
该多克隆抗体用与人类己糖激酶i氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白a和肽亲和层析纯化制得。
Antibodies are purified by protein a and peptide affinity chromatography. protein a and peptide affinity chromatography.
抗体通过蛋白a和肽段亲和色谱法纯化。
The soluble 21 peptide was purified by using chitin affinity chromatography.
经几丁质亲和层析柱纯化21肽。
The purity of inclusion bodies was above 70% and that of protein purified by nickel affinity chromatography was 95%.
提纯的包涵体纯度可达70%以上，用镍亲和层析方法纯化蛋白则可达到95%。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PRAS40. Antibodies are purified by peptide affinity chromatography.
该多克隆抗体是由合成的人源的针对PRAS40 蛋白氨基酸序列的肽段免疫动物而生产的。抗体由肽亲和层析技术纯化。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
通过固定化金属亲和层析、阴离子交换、疏水层析融合蛋白得到了纯化，其纯度可达91%。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体采用蛋白a和肽亲和层析法纯化。
The modified gene was expressed by IPTG induction and purified by affinity chromatography and cleavage in place.
IPTG诱导该基因的表达，亲和层析和原位裂解法纯化表达产物。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体由蛋白a和肽段亲和层析纯化得到。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.
而且从周质空间分离的蛋白经过镍住得到了进一步的纯化。
Heat shock protein 70 (HSP70) was purified from rat liver with DEAE ion exchange chromatography and ATP-affinity chromatography.
利用DEAE离子交换和atp亲和层析法从热休克的大鼠肝脏中分离纯化了热休克蛋白70 (HSP70)。
The GST-MBD4 fusion protein was purified from cell lysates using glutathione Sepharose 4b affinity chromatography.
用谷胱甘肽琼脂糖凝胶4b亲和介质从菌体裂解液中纯化了GST -MBD4融合蛋白。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human MEK1. Antibodies are purified by protein a and peptide affinity chromatography.
该多克隆抗体是采用合成的与人源mek1蛋白相一致的肽段免疫动物后产生的。该抗体经蛋白a和肽亲和层析纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体采用蛋白a和肽亲和色谱法纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human MEK2. Antibodies are purified by protein a and peptide affinity chromatography.
该多克隆抗体是采用合成的与人源mek2蛋白相一致的合成肽段免疫动物而产生的。该抗体经蛋白a和肽亲和层析纯化。
Results: The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography.
结果：经表达和纯化获得了纯度达90%以上的融合蛋白；
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