Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体由合成化肽段免疫动物，该合成肽段与YAP人源蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Cu(II)-immobilized metal affinity chromatography (Cu(II)-IMAC), as a highly reliable analytical procedure, has been widely used in separation and purification of biomacromolecule.
铜离子固定金属亲和色谱作为一种有效的分析方法，已普遍应用于生物大分子的分离与纯化。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
Affinity membrane separation, a new technology, has advantages of both membrane separation and affinity chromatography, and is good for the separation and purification of biological products.
亲和膜分离技术是一项新兴的技术，兼有膜分离和亲和色谱的优点，可以有效地进行生物产品的分离和纯化。
The application of molecular recognition in affinity chromatography, solid extraction, membrane separation technology raises the separation efficiency and separates those optical isomeric compounds.
分子识别应用于亲和色谱、固相萃取、膜分离技术极大地促进了分离效率的提高和一些复杂物质的拆分。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKM2. Antibodies are purified by protein A and peptide affinity chromatography.
该多克隆抗体用与人类PKM2蛋白氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CDK5. Antibodies are purified by protein A and peptide affinity chromatography.
该多克隆抗体由合成的人类CDK5氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。
Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PRAS40. Antibodies are purified by peptide affinity chromatography.
该多克隆抗体是由合成的人源的针对PRAS40 蛋白氨基酸序列的肽段免疫动物而生产的。抗体由肽亲和层析技术纯化。
Conclusion Dye affinity chromatography can be used as a refine purification method for purification of EDH and G-6-PDH from baker's yeast.
结论染料配基亲和层析可作为面包酵母乙醇脱氢酶和葡萄糖-6-磷酸脱氢酶的精制纯化方法。
The purity of inclusion bodies was above 70% and that of protein purified by nickel affinity chromatography was 95%.
提纯的包涵体纯度可达70%以上，用镍亲和层析方法纯化蛋白则可达到95%。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA. Antibodies are purified by peptide affinity chromatography.
该多克隆抗体用与人类LDHA氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白亲和层析纯化而得。
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein a and peptide affinity chromatography.
该抗体通过使用合成肽段免疫动物而获得，并经蛋白a和肽亲和层析纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human APC. Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于人源APC蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I. antibodies are purified by peptide affinity chromatography.
该多克隆抗体用与人类己糖激酶i氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白a和肽亲和层析纯化制得。
Methods Phosphoproteins were extracted from lysates of normal mice livers by phosphate metal affinity chromatography (PMAC) resin.
方法取正常小鼠肝脏，裂解肝脏后利用磷酸盐金属亲和层析(PMAC)树脂提取磷酸化蛋白。
Affinity chromatography is a powerful tool used in the purification of antibodies. In this paper, different kinds of affinity ligands used in affinity chromatography are described.
亲和色谱是用于抗体纯化的有效方法，本文对亲和色谱配体的研究进展进行了综述。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
The present invention also relates to the new method of using the new affinity chromatography mediums in purifying and producing cobra neurotoxin.
本发明还涉及用这类新的亲和层析介质纯化生产眼镜蛇神经毒素的新方法。
Purpose To study purification method of the low molecular weight heparin(LMWH) by affinity chromatography.
目的探索亲和色谱纯化低分子肝素的方法。
Methods a combination of euglobulin precipitation, ion-exchange chromatography, (NH4) 2so4 precipitation and affinity chromatography was used in the process of purification.
方法采用优球蛋白沉淀、离子交换色谱、(NH4)2so4沉淀和亲和色谱法进行纯化。
Diagnostic sensitivity and specificity of the DSA affinity chromatography of the liver-type ALP for primary hepatocarcinoma are all 100%.
用肝型碱性磷酸酶dsa强结合组分诊断原发性肝癌的灵敏度和特异度均可高达100%。
After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
包涵体蛋白经含8m尿素的结合缓冲液溶解后，用金属亲和层析法进行纯化，获得了高纯度的目的蛋白。
Objective to study the applicatory value of affinity chromatography of N-linked sugar Chain of the liver-type alkaline phosphatase in diagnosis of primary hepatocarcinoma.
目的研究肝型碱性磷酸酶n -糖链亲和层析在原发性肝癌诊断中的应用价值。
Typically, in previous reports, the purification of CP mainly relied on the antibody, liquid chromatography and affinity tag-based strategies.
在先前的研究中，CP的分离主要依赖抗体、液相色谱以及亲合标签纯化等技术。
So in this study, based on the mechanism of affinity chromatography, the affinity chromatography column with the heme immobilized was built to do purification of HBP.
根据亲和层析的原理，把血红素作为配基制备亲和层析柱用于纯化HBP，为进一步研究HBP打下基础。
Finally, it puts forward some problems in the application of affinity chromatography and forecasts its future development.
并且提出了存在的问题以及今后发展趋势。
Finally, it puts forward some problems in the application of affinity chromatography and forecasts its future development.
并且提出了存在的问题以及今后发展趋势。
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