The key of antibody-mediated immunological therapy is strong specificity of target antigen, low immunogenicity, high affinity of antibody and so on.
而抗体介导的免疫治疗的关键是靶抗原的特异性强,抗体的免疫原性低及亲和力高等。
Conclusion With the increase of the immunization times, the affinity of antibody come to mature and the proportion of dominant linear epitopes of BSA became higher.
结论抗体抗原亲和力随免疫次数的增加趋向饱和,BSA的线性表位为优势表位,随免疫次数的增加比重逐渐增强。
Because this method was to simulate the process of antibody maturation in vivo, the obtained antibodies was not only fully humanized but also with relatively high affinity.
该方法体外模拟了抗体亲和力成熟的过程,使得到的抗体不但可以完全的人源化,而且可以具有较高的亲和力。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Conclusion Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
结论错配pcr结合噬菌体展示技术是提高单链抗体亲和力的一种有效且简便的手段。
The residue activity of modified enzyme is 32.8% when the affinity to antibody disappear and the stability of modified enzyme is promoted obviously.
得到的修饰酶抗体结合能力消失,保留酶比活力32.8% ,酶的常温稳定性和热稳定性均有显著提高。
Typically, in previous reports, the purification of CP mainly relied on the antibody, liquid chromatography and affinity tag-based strategies.
在先前的研究中,CP的分离主要依赖抗体、液相色谱以及亲合标签纯化等技术。
Typically, in previous reports, the purification of CP mainly relied on the antibody, liquid chromatography and affinity tag-based strategies.
在先前的研究中,CP的分离主要依赖抗体、液相色谱以及亲合标签纯化等技术。
应用推荐