Objective :To find out a method of extraction and purification of bone morphogenetic protein (BMP) from the conditioned medium of human osteosarcoma cell, and evaluate its biological activity.
目的:探索从人骨肉瘤细胞条件培养基中提取骨形态蛋白(BMP)的方法,并测定其生物学活性。
Results: in human osteosarcoma cell line MG-63 culture Type I collagen mRNA expression reach a maximum level on days 7.
结果:人成骨肉瘤MG- 63细胞株接种培养第7天后I型胶原基因表达较高。
Results: in human osteosarcoma cell line MG-63 culture Type I collagen mRNA expression reach a maximum level on days 7.
结果:人成骨肉瘤MG- 63细胞株接种培养第7天后I型胶原基因表达较高。
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