方法：采用生长曲线和集落形成实验。
Method: the cell proliferation curve and the clone forming approaches were adopted.
通过细胞形态、软琼脂集落形成实验检测细胞的转化。
The cell growth and soft AGAR assay were run to test the transformed cells.
蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
方法软琼脂集落形成试验和裸鼠成瘤实验。
Methods Soft AGAR assay and nude mouse tumorigenicity assay were used.
并进行体外靶细胞结合实验、细胞毒性实验和集落形成抑制实验。
The two conjugates were proven to combine with osteosarcoma cell and in vitro cytotoxicity test and colony inhibition test were also performed.
与原位缺口翻译实验相对应，集落形成试验表明，在高浓度醋酸棉酚或GTW作用下，细胞失去繁殖的能力；
In a colony-forming test, we found that the cells lost their proliferate ability, since no colonies could be formed, if gossypol or GTW were of high concentration;
裸鼠处死时测量肿瘤重量，用半固体培养基进行粒—巨噬细胞集落形成单位(CFU - GM)实验并检测血白细胞、丙氨酸转氨酶和肌酐等。
When the mice were sacrificed, tumor weight was measured, and the assay of colony forming unit-granulocyte and macrophage (CFU-GM) was performed on semi-solid culture.
裸鼠处死时测量肿瘤重量，用半固体培养基进行粒—巨噬细胞集落形成单位(CFU - GM)实验并检测血白细胞、丙氨酸转氨酶和肌酐等。
When the mice were sacrificed, tumor weight was measured, and the assay of colony forming unit-granulocyte and macrophage (CFU-GM) was performed on semi-solid culture.
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