目的：评价酶免疫分析法（ELISA）体外检测过敏原在荨麻疹病因筛查中的临床应用价值。
Objective: To evaluate the clinical value of in vitro allergen detection by ELISA in aetiological screening of urticaria.
方法采用酶免疫分析法对40例复发性口腔阿弗它溃疡的患者、21例健康人进行食物过敏原的检测。
Methods 40cases with RAU and 21 health people were detected by IVT enzyme immunoassay with food allergens.
本文采用酶免疫分析法测22例缺氧缺血性脑病（HIE患儿血清中神经元特异性烯醇化酶（NSE的变化。
Serum samples taken from 22 patients with hypoxia-ischemia encephalopathy (HIE were determined with enzyme immunoassay using a kit for the measurment of NSE.
采用电化学发光免疫分析法检测血清CA125、CA153、CA199，采用微粒子酶免疫分析法检测血清scc。
Electrochemical luminescence immunoassay method was used to detect CA153, CA199 and CA125, and particle enzyme immunoassay method was used to detect SCC.
方法：采用化学发光酶免疫分析法检测5 0例重症感染新生儿及4 0例正常健康儿童血清IL6、IL8水平。
Methods: Serum IL 6 and IL 8 were measured with chemiluminescent enzyme immunoassay in 50 newborns with severe infection and in 40 healthy infants.
酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用，其灵敏度取决于标记酶的高度纯化和高转化率。
The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label.
使用免疫细胞化学法分析骨髓间充质干细胞是否表达淀粉酶抗体。
Since the acinar cell was the only cell which had the function of secreting amylase, the transdifferentiation of bone marrow cell was identified by immunocytochemical analysis with amylase.
电化学酶联免疫传感器广泛应用于临床检测、生化分析等领域。
Electrical Enzyme-linked Immunosensor (EEIS) has been widely applied to the clinical and biochemical analysis.
具有更广泛应用前景的现场快速检测技术：免疫分析、生物传感器、酶抑制技术等。
The potentiality in the use of biosensor, immunoassay and enzyme inhibition were great in the rapid detection on the spot.
近年来，分子印迹法制得的聚合物在分离、分析、免疫测定、催化、模拟酶及生物传感器等方面的应用引起人们的广泛关注。
Polymers prepared by molecular imprinting have received much attention in recent years for separations, analysis, immunoassays, catalysis, enzyme mimics and biosensor.
采用免疫组化和酶谱分析等方法，观察各组微血管结构、明胶酶及其抑制物的变化。
Immunohistochemical method and zymogram analysis method, etc. were adopted to observe the change of microvessel structure, gelatinase and its inhibitor expression.
综述了近年来常用于电化学酶联免疫传感器的标记酶及其在分析测试中的应用。
This paper deals in details with the recent development of labeled enzyme in EEIS, and the application in analytical Chemistry.
本文首次提出邻联甲苯胺（OT）-H_2O_2-辣根过氧化物酶（HRP）伏安酶联免疫分析新体系。
A voltammetric enzyme - linked immunoassay based on a new system of o- tilidine (OT) - H2O2 - horseradish peroxidase (HRP) has firstly been developed.
提出了一种基于酶催化沉积放大的电化学免疫分析方法。
A novel electrochemical immunoassay method based on enzyme-catalyzed depositing enlargement is proposed.
方法：采用放射免疫分析法和硝酸还原酶法测定20例HIE新生儿和20例正常新生儿血浆et与血清NO水平。
Methods: Plasma et levels were determined by using a radioimmunoassay kit, serum no levels were determined by using a nitrate reductase method in 20 newborn with HIE and 20 normal newborn.
方法采用固相酶联免疫分析法检测75例过敏性紫癜患者血清IL-18、VEGF水平。
Methods An enzyme-linked immunosorbent assay(ELISA)was utilized to detect the levels of IL-18, VEGF in serum of 75 patients with HSP.
目的：了解磁珠酶联免疫分析法检测人类白细胞抗原-B27的准确性、敏感性和特异性，探讨其在强直性脊柱炎辅助诊断中的价值。
OBJECTIVE: To explore the accuracy, sensitivity and specificity of IMS-ELISA for detecting HLA-B27 and its value in the auxiliary diagnosis of ankylosing spondylitis.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
方法：应用免疫放射分析(irma)法测定血清nse含量和应用酶联免疫反应(elisa)法测定血清gst含量。
Methods the serum levels of NSE in cerebral infarction patients were determined with immunoradiometric assay (IRMA), and the serum level of GST were determined by enzyme immuno sandwich assay (ELISA).
农药小分子的酶免疫化学研究可以是针对单个化合物制备特异性抗体，也可以针对一类化合物制备“簇特异性”抗体从而进行多残留免疫分析。
The enzyme immunochemistry of pesticides including the preparation of the specific antibody for single compound and the "broad specific" antibodies for multi-residue analysis.
采用光度酶联免疫分析法（ELISA）检测胰腺正常组、对照组、干预组中的LN的含量。
The Laminin(LN)level in normal group, control group and interference group was detected by using enzyme immunoassay(ELISA).
方法分别在手术，放疗或化疗前后2周内采集PLC病人血清标本162份。采用酶联免疫分析方法检测标本血清中CYFRA21 1和nse的表达情况。
Methods CYFRA21 1 and NSE in serum sample of 162 patients with PLC were analysed by ELISA in two weeks before and after treatment of operation, radiotherapy and chemotherapy.
介绍了磁性均相酶联免疫分析仪的测量原理，给出了仪器的整体设计、电路部分和软件框图。
The measurement principle of a magnetic homogeneous enzyme immunoassay analyzer is described. The design of the instrument, the circuitry and software diagram are presented.
ECL在生物传感器研究、免疫分析和DNA探针、表面分析和酶分析中得到了广泛的应用。
ECL is widely used in biosensor, immune sensor, DNA probe, surface analysis and enzyme analysis.
目的对10699名献血人员抗-TP检验结果进行分析方法采用酶联免疫法（ELISA）进行检测。
Objective To analyse anti-TP test results of 10699 blood donors. Methods Enzyme-linked immunosorbent assay (ELISA)was used.
应用酶联免疫法分析半抗原抗体滴度。
The titers were determined by enzyme linked immunosorbent assay.
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中血小板抗体水平。
This assay employs the quantitative sandwich enzyme immunoassay technique. Platelet antigens have been pre-coated onto a microplate.
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中淋巴细胞因子水平。
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for lymphocyte factor has been pre-coated onto a microplate.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
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