向细胞裂解液中加入蛋白酶抑制剂后可以保护细胞提取物中的靶蛋白。
The addition of protease inhibitors to the cell lysis buffer AIDS in the preservation of target proteins in the cell extract.
蛇毒类凝血酶属于纤维蛋白原裂解酶,是一种丝氨酸蛋白酶。
Snake venom thrombin-like enzymes belong to the fibrinogenolytic enzyme family and are serine proteases.
为便于从融合蛋白中解离出目的多肽,这个载体中含有特异的蛋白酶裂解位点。
This vector also contains thrombin protease cleavage site to facilitate proteolysis of the bacterial fusion proteins.
当细胞被裂解提取全细胞提取物时,会发生正常的细胞调节缺失,并且细胞提取物中的内源性蛋白酶可以在失控的情况下使蛋白发生降解。
A loss of normal cellular control occurs during cell lysis, and endogenous proteases within the cell extract are free to degrade proteins in an uncontrolled manner.
胰蛋白酶水解位点分析表明C肽无裂解位点,而A肽和B肽则分别具有3个和4个潜在水解位点。
No putative trypsin hydrolysis site was found in C peptide, however, 4 and 3 potential sites were found in A and B peptides respectively.
在这个实施方案中,底物为蛋白水解酶提供裂解目标。
In this embodiment, the substrate provides a cleavage target for a proteolytic enzyme.
本文建立了蛋白酶体裂解内源性抗原蛋白的QSAR模型来研究蛋白酶体对抗原蛋白裂解的特异性,并采用PLS方法求解模型。
The QSAR model of proteasomal cleaving antigen protein is built in order to study the specificity of the proteasome cleaving antigen protein, and PLS method is used to solve the model.
对其氨基酸序列进行分析,蛋白酶裂解位点序列除LH株为GKRKKR外,其余三株为RRRKKR,为高致病性禽流感病毒的特征序列;
The proteolytic cleavage sites were all hexylbasic amino acids RRRKKR, with exception of LH (GKRKKR), which were the characteristic sequence of the highly pathogenic strains;
对其氨基酸序列进行分析,蛋白酶裂解位点序列除LH株为GKRKKR外,其余三株为RRRKKR,为高致病性禽流感病毒的特征序列;
The proteolytic cleavage sites were all hexylbasic amino acids RRRKKR, with exception of LH (GKRKKR), which were the characteristic sequence of the highly pathogenic strains;
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