前言：在人类细胞遗传学中，荧光分子技术的出现带来了一些染色体研究和诊断的新方法。
Introducetion: in human cytogenetics, the advent of fluorescence molecular techniques has brought forth new procedures for chromosome investigation and diagnosis.
通过粗线期染色体荧光原位杂交技术，将发生易位的第6号和第12号染色体的易位点分别锚定在1个和3个BAC克隆库中。
Transpositional points between chromosome 6 and 12 were anchored in 1 and 3 BAC clone's pool respectively by fluorescence in situ hybridization technology of pachytene chromosomes.
端粒克隆、着丝粒克隆以及重叠群之间空缺的大小都经过粗线期染色体荧光原位杂交鉴定。
BAC clones corresponding to telomeres, as well as to the centromere position and the gap sizes between contigs, were determined by BAC-pachytene chromosome fluorescence in situ hybridization (FISH).
免疫荧光实验结果指出，去除DNA和组蛋白的细胞核和染色体与抗肌动蛋白抗体呈阳性反应。
Indirect immunofluorescence tests showed that the DNA - and histone-depleted nuclei and chromosomes reacted positively with the anti-actin antibody.
目的探讨用多色荧光原位杂交(MFISH)技术检测的易位和双着丝粒染色体畸变的差异。
Objective To explore the differences between radiation-induced translocation and dicentric chromosome aberrations detected by multicolor fluorescence in situ hybridization (M-FISH) method.
方法16个RB患者成对的肿瘤与其相应血清标本在13号染色体上14个微卫星标记处通过荧光PCR进行扩增，分析测定LOH ；
Methods Paired blood and RB tumor samples from 16 patients were analyzed with fluorescent PCR for LOH at 14 microsatellite marker loci on chromosome 13.
结论与传统的染色体显带技术相比，荧光原位杂交技术具有高效、灵敏、可靠的特点，可为临床提供良好的辅助诊断。
Conclusions Compared with the conventional chromosome banding technique, FISH has characteristics of high sensitivity and accuracy, and provides a good assist method for diagnosis.
着丝粒和端粒DNA探针多色荧光原位杂交是一种较为精确的分析微核染色体组成的方法。
Multicolor FISH with centormeric and telomeric DNA probes was a precise technique for analyzing chromosomal composition of MN.
目前我们采用荧光原位杂交(FISH)分析法对胰腺肿块细针穿刺活检(FNA)样本行染色体异常检测还没有深入研究。
Detection of chromosomal abnormalities by fluorescence in situ hybridization (FISH) analysis has not been well-studied in FNA samples of pancreatic masses.
目的探讨一种以胎盘绒毛取样进行荧光原位杂交（FISH）快速诊断染色体数目的方法。
Objective To study the numerical abnormality of chromosomes in lung cancer by dual-color fluorescence in situ hybridization(FISH).
粗线期染色体荧光原位杂交结果表明，大多数染色体的末端都有端粒串联重复，但信号的强度在不同染色体上是不同的。
Pachytene FISH results showed that most of chromosome ends possess the telomere tandem repeats, but the signals on different chromosomes were not the same in intensity.
方法采用常规染色体G 带分析和荧光原位杂交（FISH）方法对155例NHL患者的淋巴结组织进行细胞和分子遗传学研究。
Methods Routine G banding chromosome analysis and fluorescent in situ hybridization(FISH)were performed on lymph node specimens from 155 NHL patients.
目的建立多色荧光原位杂交技术检测人卵细胞染色体非整倍体的方法。
Objective: To establish the method of aneuploidy detection in human oocytes by multicolor fluorescence in-situ hybridization (M-FISH).
方法：取试管婴儿助孕技术后未能受精成功的卵细胞，采用多色荧光原位杂交方法检测卵细胞13，16 ，18，2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
方法：取试管婴儿助孕技术后未能受精成功的卵细胞，采用多色荧光原位杂交方法检测卵细胞13，16 ，18，2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
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