变形链球菌；荧光定量PCR；唾液；
采用荧光定量pcr地办法停止定量剖析。
Uses fluorescence quota PCR the method to carry on the quantitative analysis.
采用荧光定量pcr的方法进行定量分析。
Using fluorescence quantitative PCR method of quantitative analysis.
采用实时荧光定量PCR鉴定高表达基因。
Differentially expressed genes were identified by real-time quantitative PCR.
采用实时荧光定量pcr的方法进行定量分析。
Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis.
实时荧光定量pcr广泛应用于植物病理学研究的各个领域。
Real time quantitative PCR has been widely used in various areas of plant pathology research.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
构建汉坦病毒S片段标准品，用于实时荧光定量pcr检测汉坦病毒。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
并通过荧光定量PCR研究其在外施五种激素的条件下的胶乳中的表达谱。
The real time quantitative PCR is applied to research the expression trend of these genes under five different hormones.
方法根据标准质粒荧光定量PCR的扩增曲线选择合适有效的扩增循环次数；
Methods Choose correct and effective PCR cycle number according to standard plasmid's amplification curve in real-time PCR;
目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的运用终点法荧光定量pcr检测HBV -DNA，探讨其临床应用价值。
Objective To investigate the clinical value of endpoint detection of HBV-DNA by means of fluorescent quantitive PCR.
这说明荧光定量RT-PCR方法可以对临床上H5N1虎源流感病毒检测提供参考。
The result indicated that fluorogenetic quantitation RT-PCR may be used as a good reference in detection of H5N1 TIV.
荧光定量方法的建立：用异硫氰酸荧光素成功对白蛋白和纤维蛋白原进行了荧光标记。
Establish the quantitive fluorimetry: Bovine serum albumin (BSA) and fibrinogen (Fg) was successfully labeled with FITC.
目的：用荧光定量聚合酶链反应(FQ - PCR)测定丙型肝炎病毒在临床的应用。
Objective To investigate the clinical application of the Fluorescence quantitative PCR (FQ-PCR) in the detection of HCV infection.
前言： 目的：评估荧光定量PCR在检测胃粘膜上幽门螺杆菌的DNA中的应用价值。
Objective:To evaluate the clinical application value of FQ-PCR(fluorogenic quantitative polymerase chain reaction) for detecting HP DNA in human gastric mucosa.
目的探讨实时荧光定量pcr检测巨细胞病毒在肾移植术后肺部感染诊断中的应用价值。
Objective to examine the value of real time PCR in the diagnosis of cytomegalovirus pneumonia in renal Allograft Recipient.
建立一种利用荧光定量pcr扩增反应进行单核苷酸多态性(snp)快速测定的方法。
A new effective single nucleotide polymorphism (SNP) typing method was developed by using real-time polymerase chain reaction (PCR).
目的探讨荧光定量聚合酶链反应(FQ - PCR)在巨细胞病毒性肝炎中的应用价值。
Objective To investigate the fluorescence quantitative polymerase chain reaction (FQ-PCR) in the giant cell viral hepatitis in value.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
目的：制备h BV - DNA荧光定量pcr检测的室内质控物，并对其应用价值进行初步评价。
Objective: to develop an internal quality control substance of HBV-DNA by real time quantitative PCR and to evaluate its clinical value.
目的：探讨脂血、高胆红素和溶血标本对乙肝病毒DNA（HBVDNA）荧光定量测定结果的影响。
Objective: To investigate the effect of the high lipid, high bilirubin and hemolytic blood specimens on real time fluorimetry PCR of HBVDNA.
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
目的探讨荧光定量pcr (FQPCR)检测细胞角蛋白19 (ck19)在乳腺癌诊断中的应用。
Objective to evaluate the clinical application of fluorescent quantification polymerase chain reaction (FQ PCR) to detect cytokeratin 19 (CK19) in the diagnosis of breast cancer.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
目的探讨用荧光定量PCR检测不明原因流产组织及血清中巨细胞病毒（CMV）和弓形体（TOX）感染。
Objective: Detect the infection of CMV and TOX in tissue , serum of unexplained abortion by FQ-PCR.
分别采用流式细胞计数仪和荧光定量PCR法检测慢性乙型肝炎患者外周血淋巴细胞凋亡率与血清HBVDNA。
The apoptosis of peripheral lymphocytes extracted from chronic hepatitis B patients were analyzed by flow cytometer. Serum HBVDNA was detected by quantitatively PCR.
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