我们从四个健康的供体分离人脂肪间充质干细胞。
We isolated human adipose tissue-derived MSC from four healthy donors.
脂肪间充质干细胞和骨髓间充质干细胞表面表达CD 29、CD44，不表达CD 34。
Bone marrow mesenchymal stem cells and adipose mesenchymal stem cells expressed CD29 and CD44 positive, CD34 negative.
近年研究表明，运用相似的分离方法，可从人、小鼠、大鼠、兔和猪等物种脂肪组织中分离获得脂肪间充质干细胞。
Recent researches have demonstrated the potential ability of adipose mesenchymal stem cells (AMSCs) which were harvested from human, mouse, rat, rabbit and pig adipose tissue, respectively.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
普通的间充质干细胞有多种来源(包括骨髓)，但产量原没有脂肪组织中得到的多。
Normal mesenchymal cells can be isolated from various sources, including bone marrow, but the yield is not nearly as great as what the researchers derived from fat tissue.
由于脂肪组织与骨髓同为中胚层起源的组织，那么能否从脂肪组织中获得间充质干细胞，而且也具有多分化潜能呢？
Because both fat tissue and bone marrow are tissues originated from mesoderm, so whether MSCs could be obtained from fat tissue as well, which also have multi - lineage differentiation potential?
最近出现了一种趋势，利用来自骨髓和脂肪间充质的干细胞。
Recently there has been a trend towards the use of mesenchymal stem cells from bone marrow and fat.
本文综述了脂肪来源间充质干细胞的分离培养、生物学特性及其应用等进展，并对其研究前景进行了探讨。
Based on the progress in this field, this review focuses on ADSCs' isolation, culture, biological characteristics and applications, as well as their research prospect.
本发明公开了一种诱导间充质干细胞和脂肪干细胞分化为神经元的方法及其专用培养基。
The present invention discloses a method for inducing mesenchymal stem cell to divide into nerve cell and the special culture medium.
来自人脂肪组织的间充质干细胞与骨髓间充质干细胞是一类具有非常相似表型的细胞群。
MSC from human bone marrow and adipose tissue represent very similar cell populations with comparable phenotypes.
微流控芯片也被用于研究骨髓间充质干细胞的培养及相应的成脂肪细胞和神经细胞的分化。
The results show that even though the culture of stem cells can be done with different substrates, the differentiation is more critically depending on the cell-material adhesion.
结论：蚕丝蛋白生物支架具有良好的三维空间结构和生物降解性，人脐带间充质干细胞接种于蚕丝蛋白生物支架可用于组织工程化脂肪组织的研究；
Conclusions The silk fibroin scaffold is a new type of scaffold with 3D space structure and good biodegradation. hUCMSCs could be used in the research of tissue engineering adipose.
结论：蚕丝蛋白生物支架具有良好的三维空间结构和生物降解性，人脐带间充质干细胞接种于蚕丝蛋白生物支架可用于组织工程化脂肪组织的研究；
Conclusions The silk fibroin scaffold is a new type of scaffold with 3D space structure and good biodegradation. hUCMSCs could be used in the research of tissue engineering adipose.
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