方法：用胰酶消化法培养兔rpe细胞并传代。
Methods: The rabbit RPE cells were cultured with trypsin enzyme-digesting technique and passaged in vitro.
方法：用胰酶消化法获取人胎儿视网膜色素上皮细胞进行原代及传代培养。
Methods: The fetal RPE cells were cultured with trypsin enzyme digesting technique and passaged in vitro.
表明利用胰酶消化法可以分离培养出纯度高，数量较多且培养时间明显缩短的成骨细胞。
Tryptic digestion could isolate and culture Numbers of osteoblasts with a high purity and obviously shorten the culture time.
方法：采用胰酶消化法分离小鼠胚胎第8 . 5天的颅神经管，从小鼠颅神经管中游离出来的细胞即为颅神经嵴细胞。
METHODS: cranial neural tubes were dissected from 8.5 day mouse embryo using trypsin digestion and explanted in culture dishes. Cells dissociated from the neural tubes were cranial neural crest cells.
采用冷胰酶消化法分离培养大鼠睾丸支持细胞，采用单细胞凝胶电泳法检测亚硝酸钠对大鼠睾丸支持细胞dna的损伤作用。
Method: The effects of sodium fluoride on DNA damage and repair in isolated male mice testicular cells were observed with single cell gel electrophoresis test (Comet assay).
方法取大鼠胚胎分离成纤维细胞，分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
结论胰酶消化植块培养法是一种能够获得高成活率、高收获量、细胞形态和生长状况良好的体细胞培养方法。
Conclusions method of trypsinization is good method which acquire high percent of live cells and more production of cells.
结论：胰酶消化、差速贴壁法是一种适合的心室成纤维细胞的培养方法。
Conclusion: Trypsin enzymic digestion and differential attachment was suitable for culture of ventricular fibroblast.
方法：组织块反复种植纯化法、低含量胰酶快速消化和差速贴壁法。
METHODS: The repeated implantation, rapid trypsinization, and differential adhesion were used.
方法：组织块反复种植纯化法、低含量胰酶快速消化和差速贴壁法。
METHODS: The repeated implantation, rapid trypsinization, and differential adhesion were used.
应用推荐