方法：分别用胰蛋白酶消化法及胃蛋白酶-胰蛋白酶联合消化法制备视网膜血管铺片，比较铺片成功率。
METHODS: Retinal vascular preparations were performed by using trypsin digestion and pepsin-trypsin digestion technique respectively.
以18天鸡胚睾丸为实验材料，经胶原酶和胰蛋白酶两步酶消化法得到生精上皮细胞悬液。
Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
方法：采用分离酶和胰蛋白酶联合消化法分离小型香猪角质形成细胞，角质形成细胞培养基培养。
METHODS: Keratinocytes of mini fragrant pigs were separated by dispase and trypsin combined digestive method, and then cultured with keratinocyte culture medium.
用胰蛋白酶和胶原酶消化法和组织贴块法培养猪肺动脉干内皮细胞和平滑肌细胞。方法简便易行，成功率高。
Endothelial cells and smooth muscle cells of porcine pulmonary trunk were cultured by enzyme-dispersed and substrate-attached explant methods.
用胰蛋白酶和胶原酶消化法和组织贴块法培养猪肺动脉干内皮细胞和平滑肌细胞。方法简便易行，成功率高。
Endothelial cells and smooth muscle cells of porcine pulmonary trunk were cultured by enzyme-dispersed and substrate-attached explant methods.
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