三维细胞培养方法的综合评述。
改进体外成肌细胞培养方法。
改良组织块法是一种良好的大鼠牙囊细胞培养方法。
Improved explant culture was a good culturing method for rat DF cells.
方法：采用国际通用的微量淋巴细胞毒试验和混合淋巴细胞培养方法；
Methods:The conventional microlymphocytotoxicity test and the lymphocyte co cultivation were used.
方法用流行病学观察法进行监测。实验用人胚肾细胞培养方法分离病毒。
Methods Epidemiological observation and experiment of isolating virus through culture of human embryo kidney cell were used in this study.
我们采用双峰驼的外周血液淋巴细胞培养方法，对其染色体进行了分析研究。
The chromosomal complement of Bactrian Camel has been studied by means of peripheral blood lymphocytes culture.
方法采用外周血淋巴细胞培养方法制备染色体，G、C等显带技术进行细胞遗传学分析。
Methods: Routine culture for the peripheral blood lymphocytes were made and G, C band were analysed.
方法抽取104例孕16～29周孕妇的羊水，采用改良的羊水细胞培养方法进行培养。
Methods 104 samples of amniotic fluid from pregnant women during 16-29th-week gestation were cultured and karyotyped.
目的建立螨类细胞培养方法，为从细胞分子水平研究革螨、恙螨作为HFRS媒介提供条件。
Objective to establish cell culture method of the gamasid mites in order to study on the gamasid mites and chigger mites as the vectors of HFRS at the cell molecular level.
前言：目的：探讨高效的原代成年大鼠下颌骨成骨细胞培养方法，为进一步的实验研究奠定基础。
Objective: To investigate an efficient method of primary mandibular osteoblasts culture, and establish a foundation for further experimental study.
采用外周血淋巴细胞培养方法和常规染色体制备技术，对内蒙古乌审马染色体核型进行了分析研究。
In this paper, chromosomal karyotype of Wushen horse in Inner Mongolia was studied by using the peripheral blood lymphocytes culture method and conventional chromosome analysis techniques.
目的应用EBM - 2培养犬脐血间充质干细胞(msc)，为MSC的研究提供新的细胞培养方法。
Objective to culture mesenchymal stem cells (MSC) in cord blood of dogs with EBM-2, providing a new method to culture cells for research in MSC.
结论胰酶消化植块培养法是一种能够获得高成活率、高收获量、细胞形态和生长状况良好的体细胞培养方法。
Conclusions method of trypsinization is good method which acquire high percent of live cells and more production of cells.
结论酶交替消化法原代培养胎鼠成骨细胞，获得的成骨细胞纯度高、数量大，可作为一种相对可靠、有效的原代成骨细胞培养方法。
Conclusion Osteoblasts achieved by this method are pure with large quantities, so this method can be used as a reliable and efficient way of primary culture.
利用生物化学和细胞培养方法对研制出的新型羧甲基壳多糖微载体CX-2的生物亲和性、细胞贴壁及生长和扩展特性进行了研究。
The properties of the newly developed microcarrier (MC) CX-2 were investigated using biochemical and cytological techniques, including bio-affinity, cyto-compatibility, eurytopicity, and so on.
目的在本实验室建立小鼠胚胎成纤维细胞培养方法，并在此基础上用培养的细胞检测氯化镉、甲醛等外来化合物的细胞毒性反应。
Objectives: To set up the methods of mouse embryo fibroblasts culture in vitro and to evaluate the toxicity of xenobiotics such as chloride cadmium and formaldehyde by using these methods.
方法采用细胞培养技术、免疫细胞化学方法观察新生大鼠皮层神经干细胞向胆碱能神经元定向分化的时空变化。
Observation the space-time change of cortex nerve stem cell in newborn rat orientation differentiation to cholinergic neuron by cell cultured and immunocyte chemistry methods.
方法：采用细胞培养法。
方法：采用细胞培养技术和组织工程学方法。
METHODS: Cell culture technique and tissue engineering method were used.
方法采用体外细胞培养计数法，结果表明，该多糖对胃癌有显著性抑制作用。
Methods in vitro culture cell number count was adopted. Results AGP significantly inhibited the gastric cancer cell.
方法采用体外细胞培养计数法。
Methods: In vitro culture cell number count assay was adopted.
方法应用体外细胞培养技术观察MC3T3 - E1成骨细胞株在不同处理的牙骨质表面的生长状况。
Methods Cell culture technique in vitro had been used to study the growth condition of MC3T3-E1 osteoblast line on the surface of cementum treated with various methods.
方法用酶消化、分离鸡胚关节软骨细胞，体外单层培养，收集细胞培养液。
Methods Chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro. Chondrocyte culture media was collected.
方法将人肝门部胆管癌标本进行细胞培养，传代。
Methods The fresh human hilar cholangiocarcinoma specimen was cultured.
目的：研究外周干细胞培养液的细菌内毒素检查方法。
OBJECTIVE: to investigate bacteria endotoxin test for peripheral blood stem cells culture medium.
冷冻保存方法是：将表皮细胞培养膜片固定在含硅油纱布上进行冷冻保存(方法a)和将在培养瓶中的表皮细胞培养膜片直接冷冻保存(方法b)。
Method a: Cultured epidermal cell sheets were fixed on the silicone oil gauze. Method b: Direct cryopreservation of the culture chamber containing epidermal cell sheets.
通过愈伤组织培养、悬浮细胞培养和原生质体培养方法对草坪草的一些种已建立较为完善的植株再生体系。
The plant regeneration systems of some turfgrass species have been established perfectly through callus culture, cell suspension culture, and protoplast suspension culture.
方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
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