方法：应用免疫组化法检测乳腺癌及相应癌旁组织tlr4、增殖细胞核抗原(pcna)的表达，TUNEL法检测组织中细胞的凋亡。
Methods: the expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method.
肺泡巨噬细胞(AM)表面CD 14表达测定采用免疫组化法，并进行计算机图像分析。
The expression of CD14 on alveolar macrophage (AM) surface was assayed by immunohistochemistry and then analyzed with computer imaging system.
免疫组化方法观察电针组星形胶质细胞BDNF的表达，MTT法检测电针血清对星形胶质细胞活力的影响。
Immunohistochemical method was introduced to observe the BDNF expression of astrocytes in electrical needle group, MTT method to examine the effect of serum on the astrocytes activity.
采用免疫组化法检测各组大鼠支气管上皮细胞ICAM -1和HO - 1表达。
The expression of ICAM 1 and ho 1 on broncho epithelial cells were detected through immunohistochemistry method.
免疫组化法观察细胞周期调控蛋白的表达变化。
The expression of protein related cell cycle was observed by immunohistochemical.
AB PAS特染法检测哮喘小鼠小支气管杯状细胞的分布；同一解剖部位采用免疫组化染色法检测GMCSF的表达。
The goblet cells in small bronchi were identified with AB-PAS staining, and the expression of GM-CSF in the same airway was assessed with immunohistochemistry staining.
方法：ABC免疫组化法及流式细胞术。
Methods: ABC immunohistochemical technique and flow cytometry were used.
方法运用SP免疫组化法，检测52例经病理证实的星形细胞瘤瘤内MVD和VEGF表达。
Methods The MVD and expression of VEGF were examined by means of SP immunohistochemical technique in 52 cases of astrocytoma proved by pathology.
方法回顾性分析30例HNL患者淋巴活检标本的HE切片，并用免疫组化S- P法检测细胞表型。
Methods HE sections of lymph node biopsies of 30 patients with HNL were analyzed retrospectively and their cell phenotypes were examined by immunohistochemistry S-P method.
术后取右下肺组织活检标本，观察肺血管内皮组织形态学改变，冰冻切片免疫组化法检测肺血管内皮细胞粘附因子(ICAM)1的表达。
Lung biopsy specimens were obtained at end of operation in order to study histological changes of lung vascular endothelium. The expression of ICAM 1 on lung vascular endothelium was detected.
方法：采用免疫组化（SP法）和细胞图像分析技术。
Method:By using immunohistochemical method(SP) and image analysis technique.
用双染免疫组化法检查脾细胞中STAT 5磷酸化与STAT 5抗原的定位。用电泳迁移率变动分析(EMSA)测定STAT 5与DNA探针的结合力。
The phosphorylation of STAT5 and its location were examined by double immunohistochemical staining and the binding of STAT5 with DNA was determined by electrophoretic mobility shift assay (EMSA).
方法通过TUNEL法检测三类乳腺癌中凋亡细胞数量及分布比例的差异，采用S-P免疫组化法检测癌周淋巴细胞中CD57抗原表达强度的差异。
Methods The number of apoptosis cells were identified by TUNEL method and the expression intensity of antigen CD57 in lymph cells nearby the carcinoma was determined by S-P method.
免疫组化法检测各组心肌组织中CD 4 +，CD 8 +淋巴细胞数。
The CD4 + and CD8 + lymphocytes in the myocardium were counted by immunohistochemistry.
方法：用免疫印迹法检测aif在细胞中的表达量；用免疫组化法检测aif在29例胃癌组织和10例癌旁正常胃组织中的表达。
Immunohistochemical method was used to detect the expression of AIF in 29 cases of gastric cancer tissues and 10 cases of normal gastric mucosa.
应用免疫组化LSAB法，对34例尸检心脏标本，进行心肌细胞内纤维连接蛋白用于早期心肌梗死死后诊断的研究。
Immunohistochemical study of fibronectin (FN) was first per formed on 34 autopsy hearts for postmortem diagnosis of early myocardial infarction with LSAB-method.
应用抗人白细胞介素8 (IL - 8)多克隆抗体和免疫组化abc法，观察IL - 8样免疫反应阳性细胞在大鼠脑和消化道内的分布。
Interleukin 8 (IL 8) antigen in brain and gastrointestinal tract of rats was detected by using rabbit polyclonal IL 8 antibody and immunohistochemical ABC method.
方法：应用SP免疫组化法，检测P 21, P27等细胞周期依赖性激酶抑制剂和增殖细胞核抗原(pcna)在角膜上皮不同部位的表达情况。
METHODS: the expressions of CKI, P27, P21 and proliferating cell nuclear antigen (PCNA) were tested in different regions of corneal epithelium by SP immunohistochemistry.
方法免疫组化s - P法检测基质金属蛋白酶MMP - 2在成釉细胞瘤中的表达和分布。
Methods to detect the expression and site of matrix metalloproteinase-2 (MMP-2) in ameloblastoma suing S-P immunohistochemistry.
方法采用免疫组化之SP_TM法，对20例口腔粘膜白斑，20例口腔鳞状细胞癌和10例正常口腔粘膜进行染色。
Methods By immunohistochSP _TM method, staining 20 cases oral leukoplakia, 20 cases squamous cell carcinoma and 10 cases normal mucosa.
免疫组化法检测海马水平切片GFAP表达，对海马CA 1区锥体神经元进行细胞内记录。
Immunohistochemistry analysis was used to detect the GFAP expression in the dentate gyrus and CA1 area. Conventional intracellular recordings were made from the hippocampal CA1 pyramidal neurons.
免疫组化法计数VEGF阳性细胞百分数并进行半定量分级。
The VEGF positive cells percentage and semi quantitative classification were obtained by immunohistochemical staining after tumor formation in nude mice.
并将外源基因alb亚克隆入该调控序列内，转染c 127细胞，经免疫组化法检测其表达情况。
Subclone the exogenous gene ALB into the regulating sequence for transfection of C127 cells.
本文用免疫组化双PAP法研究人胚胎小肠EC细胞的形态发生。
Morphogenesis of EC cells of the human fetal small intestinal epithelium has been studied with immunohistochemical double PAP method.
方法应用免疫组化和RT-PCR法，以正常皮肤、正常疤痕为对照，检测增殖性疤痕中vegf表达，并研究VEGF与表皮细胞增殖、血管新生的相关关系。
Methods VEGF protein secretion and VEGFmRNA expression were detected by immunohistochemistry and RT - PCR, and were compared with cell proliferation and revascularization.
方法：以3 2例ACC、7例正常腮腺及3例腺泡细胞癌标本为研究对象，采用免疫组化法对NT -3进行检测。
Methods: NT-3 was examined by immunohistochemical analysis in 32 cases of salivary ACC, 7 normal parotid glands and 3 acinic cell carcinoma tissue samples.
天后收集巨噬细胞，用免疫组化法证实。
After 4 days, macrophages were collected and confirmed by immunohistochemistry.
方法采用免疫组化法对5 3例胰腺癌患者进行IL2R及T细胞定量研究。
Methods IL 2R and T lymphocytes in 53 cases with pancreas carcinoma were examined quantitatively by immunohistochemical method.
方法采用免疫组化SP法和图像分析技术对8例正常肾上腺组织20例肾上腺嗜铬细胞瘤中npy和VIP进行检测。
Methods NPY and VIP were detected by immunohistochemical technique (SP method) and image analysis in 8 cases of normal adrenal, 20 cases of adrenal Pheochromocytoma.
方法采用免疫组化SP法和图像分析技术对8例正常肾上腺组织20例肾上腺嗜铬细胞瘤中npy和VIP进行检测。
Methods NPY and VIP were detected by immunohistochemical technique (SP method) and image analysis in 8 cases of normal adrenal, 20 cases of adrenal Pheochromocytoma.
应用推荐