目的研究不同剂量X射线照射对EL4淋巴瘤细胞周期进程及细胞倍增时间的影响。
In the present study, the effect of X rays on el 4 cell doubling time and cell cycle progression were investigated.
检测了细胞倍增时间和IL - 6、趋化因子等细胞因子，从倍增时间和因子产生能力看，各克隆差异很大。
None of the cloned cells were keratin negative. The double time and IL-6 as well as chemokine production varies among different clones.
用MTT法测定生长曲线，发现与不加药的对照组细胞相比，加药组细胞生长速率均明显降低，细胞倍增时间延长，增殖受到抑制。
We measured the cells growth curves by the MTT assay. Compared with the control cells, the treated cells showed the marked growth rate decrease and the delay in double time of proliferation.
结果卵圆细胞在培养过程中倍增时间逐渐缩短，非整倍体染色体数目增加，在软琼脂中生长的克隆增多。
Results During long term cultivation, the population doubling time of the cells became shorter and the percentage of aneuploidy chromosomes and colonies grown in soft AGAR increased.
与阴性对照组比较，阳性对照组和实验组细胞群体倍增时间增加。
Compared with negative control group, the doubling time of both positive control group and experimental groups was prolonged.
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
台盼蓝排斥法进行细胞计数，绘制细胞生长曲线，计算细胞生长的倍增时间、细胞生长率及电场对细胞的杀伤率；
The trypanblue rejection method was used to take counts of the number of living cells and death ones and the growth curves was plotted.
台盼蓝排斥法进行细胞计数，绘制细胞生长曲线，计算细胞生长的倍增时间、细胞生长率及电场对细胞的杀伤率；
The trypanblue rejection method was used to take counts of the number of living cells and death ones and the growth curves was plotted.
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