另外一种较为复杂的技术，则是把胚胎细胞培养成各式各样的细胞种类及组织。
Another, more complex, technique takes embryo cells and develops them into a wide variety of cell types and tissues.
方法：采用细胞培养技术和组织工程学方法。
METHODS: Cell culture technique and tissue engineering method were used.
方法6个喉癌细胞系和4例喉癌旁正常组织上皮细胞培养。
METHODS Epithelial cell culture from 6 laryngeal carcinomas and 4 normal laryngeal tissues were conducted.
方法取良性甲状腺腺瘤手术中的腺瘤旁正常甲状腺组织进行细胞培养。
Methods the human thyroid epithelium cells (TEC) from para-adenoma normal tissues of patients with thyroid adenoma were cultured.
令人惊讶的是，根据这项新的研究，用未感染过的脑组织均浆包被的金属丝也能够在细胞培养中引发感染性朊蛋白疾病，并可使小鼠感染。
Surprisingly, according to the new research, wires coated with uninfected brain homogenate could also initiate prion disease in cell culture, which was transmissible to mice.
目的：对野生肉苁蓉及其组织细胞培养物进行润肠通便、抗疲劳功能的比较研究。
Objective:To compare the Runchang purge and anti-fatigue effects of Cistanche deserticola and the tissue culture of Cistanche deserticola.
有鉴于此，本文探讨了不同影响因子对组培桑苗中DNJ含量的影响，旨在为以后通过组织或细胞培养生产DNJ及探讨DNJ的合成机理提供参考。
In this study some factors associated with the contents of DNJ were investigated to provide a better understanding for the produce of DNJ by tissue or cell culture and the synthesis mechanisms of it .
此外，讨论了用组织培养方法生产左旋多巴的可能性和固定化细胞培养等问题。
The possibility of the production of L-dopa by tissue culture of Mucuna and the problems in immobilized cell culture were discussed.
目的：探讨利用旋转式细胞培养系统（RCCS）在体外快速构建组织工程骨的可行性。
Objective: To explore the possibility of rapid construction of tissue engineered bone in vitro by RCCS.
结论：作者通过细胞培养和组织工程学的方法，建立了小型香猪角质形成细胞库。
CONCLUSION: The fibroblasts bank of mini fragrant pigs construct by using cell culture technique and tissue engineering method.
采用组织培养和单层细胞培养两种方法对SD大鼠的胰腺进行培养。
The tissue culture and the single layer cell culture techniques for SD rat pancreatic islets were studied.
在中药治疗骨质疏松症的药物筛选，骨髓基质细胞培养与组织工程人工骨研究。
In Chinese medicine the treatment of osteoporosis drug screening, bone marrow stromal cell culture and tissue engineering artificial bone research.
通过愈伤组织培养、悬浮细胞培养和原生质体培养方法对草坪草的一些种已建立较为完善的植株再生体系。
The plant regeneration systems of some turfgrass species have been established perfectly through callus culture, cell suspension culture, and protoplast suspension culture.
植物组织或细胞培养结合理化因素的处理，可大大提高变异的范围和频率。
The techniques, developed in 1970, for plant cell and tissue culture provide the selection of resistant varieties with an advanced method.
结果：不同类型的生物反应器及其机械性能、传质以及流体剪应力等对细胞培养组织的形态和功能产生的影响不同。
Results The bioreactors with different mechanical properties, mass transfer and fluid shear stress had different impacts on morphological and functional organizations.
资料选择：选择与角膜缘干细胞组织学、生理病理学研究的相关及自体角膜缘干细胞培养移植相关文献进行初审。
STUDY SELECTION: it is to examine initially the papers relevant with studies on histology and physiopathology of limbal stem cells and relevant with autotransplantation of cultured limbal stem cells.
方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
结论采用无饲养层细胞培养体系构建自体角膜上皮组织，移植后可以成功修复角膜缘干细胞缺失的角膜上皮。
Conclusion the reconstructed rabbit corneal epithelium hi vitro with a free-feeding layer culture system could be autografted to repair total corneal limbal stem cells deficiency.
方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
结论：旋转式细胞培养系统是体外快速构建组织工程化骨的理想方法。
Conclusion: RCCS may be an ideal method for rapid construction of tissue engineered bone in vitro.
目的采用无饲养层细胞培养体系构建家兔角膜上皮组织，自体移植后观察其对角膜缘干细胞缺失的治疗效果。
Objective to reconstruct rabbit corneal epithelium in vitro with a free-feeding layer culture system and autograft to treat limbal stem cells deficiency.
采用比色法检测血清、淋巴细胞培养液和肌肉组织中的NO和NOS。
Colorimetry was used to detect the levels of NO and NOS in the serum, lymphocytic culture liquid and muscular tissue.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
选用新生SD大鼠的髁状突软骨作为原代细胞培养的组织来源，建立髁状突软骨细胞有限细胞系；
The primary MCC separated from the mandibular condylar cartilage of six neonatal SD rats were cultured and a finite cell line was established in vitro.
方法：体外试验采用组织细胞培养法，观察不同浓度药物对流感病毒FM1病毒株致细胞病变（CPE）的作用；
Method: The method of cell and tissue culture was utilized to carry out an observation on the cytopathy effect(CPE) led by influenza virus FM1 with the aerosol of different concentrations in vitro.
最初，实验人员把组织碎片放在凝胶上，浸泡在一种典型的细胞培养成分胎牛血清里。
Initially, the researchers placed the fragments on a gel and soaked them in fetal bovine serum, a typical ingredient of cell cultures.
结果两种方法均能得到乳腺癌原代细胞，组织块培养法的成功率为90.0%，细胞培养法的成功率为20.0%。
Results the breast carcinoma cells could be cultured by these two methods with a success rate of 90% in the tissue culture method and that of 20% in the cell culture method.
在植物体细胞组织培养中，蔗糖一直作为标准碳源，然而其他糖类物质如：葡萄糖、麦芽糖和山梨醇对植物体细胞培养也产生了一定的影响。
In somatic culture, sucrose has been a standard carbon source, however other kinds of sugar such as glucose, maltose and sorbitol have a certain influence on in vitro tissue culture.
在植物体细胞组织培养中，蔗糖一直作为标准碳源，然而其他糖类物质如：葡萄糖、麦芽糖和山梨醇对植物体细胞培养也产生了一定的影响。
In somatic culture, sucrose has been a standard carbon source, however other kinds of sugar such as glucose, maltose and sorbitol have a certain influence on in vitro tissue culture.
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