结论：成功地建立了人乙酰肝素酶稳定表达细胞株，为其抑制剂的筛选及其功能的研究奠定了基础。
Conclusion: Heparanase stable expression cell lines have been successfully established, which could be of great benefits to the screening and function study of its inhibitors.
目的：建立稳定表达nt3的NIH3T3细胞株，通过体外共培养实验观察其对耳蜗螺旋神经节细胞生长的影响。
AIM: to establish a NIH3T3 cell line which expresses human NT3 and to observe the growth of cochlear ganglion cells by co culturing.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
结论建立稳定表达人MUC1T739小鼠膀胱癌细胞株和t739荷瘤小鼠模型，可用于肿瘤疫苗研究。
Conclusion T739 mouse bladder cancer cell lines and tumor-bear model expressing human MUC1 were successfully established for the development of tumor vaccines targeting MUC1.
用新霉素类似物(G418)筛选出阳性克隆，并用流式细胞术进一步筛选出高效表达克隆，建立稳定高效表达CHO细胞株。
The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.
在4株转染的COS7细胞株中有2株同样具有高效稳定的TIF3编码蛋白质表达。
Similarly, 2 out of 4 COS7 cell lines had significant over expression of TIF3 encoded protein.
结论：应及时用MTX对细胞株进行再加压筛选以及保存，以保持细胞稳定表达抗体的能力；
Conclusion: MTX selection should be timely used to overcome the instability of the expression of anti HBsAg in CHO HB2 cells.
结论：在非肾脏上皮细胞获得了稳定质膜定位表达UT - A2的FRT细胞株，该细胞株可用于UT - A 2抑制剂的筛选。
CONCLUSION: We constructed an FRT cell line that could stably express UT-A2 in plasma membrane in the non-renal epithelia cell. The cell line will be used to screen UT-A2 inhibitors.
方法构建了不同TPR串联簇的截断突变体的表达质粒后，建立了稳定转染的BEL740 2细胞株。
Methods the plasmids which can express the truncated mutants of the TPR tandem clusters at different region of CRLP were constructed, stable transfected BEL7402 cell lines were established.
结论成功构建稳定沉默CXCR4表达的肾癌a 498细胞株，转染后的细胞凋亡率升高，体外侵袭能力降低，为后续研究奠定了基础。
ConclusionWe have successfully established an A498 cell line with CXCR4 stably silenced; the cell line has a higher apoptotic rate and lower invasion ability, which paves a way for future research.
结论成功构建稳定沉默CXCR4表达的肾癌a 498细胞株，转染后的细胞凋亡率升高，体外侵袭能力降低，为后续研究奠定了基础。
ConclusionWe have successfully established an A498 cell line with CXCR4 stably silenced; the cell line has a higher apoptotic rate and lower invasion ability, which paves a way for future research.
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