你引用的规则是用于覆盖全范围梯度洗脱的（例如，有机溶剂从5改变到100%），并且所指的体积是以初始条件来重新平衡。
The rule that you quoted is intended to cover a full-range gradient (for example, 5–100% organic solvent) and refers to the amount of volume to reequilibrate at the initial conditions.
采用溶剂萃取法和柱色谱法相结合的工艺，从大豆油脚中制备肌醇磷脂，研究了不同洗脱剂对柱色谱分离脑磷脂和肌醇磷脂效果的影响。
The soybean phosphatidylethanolamine and phosphatidylinositol were prepared by combining methods of solvents extraction and silica gel column chromatography from soybean oil sediment.
基于线性溶剂强度模型，应用特征线分析的方法求解梯度洗脱模式下的理想液相色谱模型。
The ideal model of liquid chromatography in gradient elution was solved by using the characteristic approach on the assumption of a linear solvent strength model.
该方法与单一溶剂洗脱相比，增加了固定相对磷脂的选择性，而且在终产物中无毒性物质残留。
Compared with the method of single-eluting solvent, this method increased the selectivity of fixed phase to phospholipid, moreover there are no toxic residue in the final products.
先固定细菌并使之在紫色染料中着色，然后再用有机溶剂如丙酮或乙醇洗脱细菌上的染料。
The bacteria are first killed and stained with the violet dye, then treated with an organic solvent such as acetone or ethanol.
再用乙醇作溶剂洗脱辣椒红素，脱除溶剂得到红色油状辣椒红素产品，色价为150。
Capsorubin was obtained from the same column using ethanol as solvent and its color number is 150.
本文综述了高温条件下，温度对柱效、保留值、选择性、溶剂洗脱特征及色谱柱稳定性的影响，介绍了高温色谱的应用进展。
The effects of temperature on column performance, retention, selectivity, and column stability are reviewed and applications of HTLC are introduced in this paper.
洗脱液以常规的顺序更换，首先用非极性溶剂，然后逐步被极性较强的所替换。
The eluent is changed in a regular manner starting with a nonpolar solvent and gradually replacing it by a more polar one.
洗脱液以常规的顺序更换，首先用非极性溶剂，然后逐步被极性较强的所替换。
The eluent is changed in a regular manner starting with a nonpolar solvent and gradually replacing it by a more polar one.
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