建立永生化细胞系是生物学研究的热点和难点之一。
Establishment of immortalized cell line is one of the hot and difficult subjects in cell biology.
建立永生化细胞系是解决体外培养条件下细胞衰老的一种常用手段。
The establishment of immortalized cell lines is a common method to resist cell aging under the condition of in vitro culture.
为了研究食管永生化细胞在不同的培养基中，培养后细胞形态的改变。
To study the change of SHEE cellular morphology passing by different culture medium.
目的研究EZH2蛋白在食管上皮永生化细胞系(SHEE)和恶性转化细胞系(SHEEC)中的表达。
Objective To investigate the expressions of EZH2 protein in the human immortalized esophageal epithelial cell line (SHEE) and the malignant transformation cell line (SHEEC).
端粒酶激活是细胞永生化和肿瘤形成的关键步骤，其活性受到多种因素的调控。
Activation of telomerase is the critical step in cellular immortality and oncogenesis. Its activity is controled by many factors.
具有软骨细胞表型特征的永生化兔髁突软骨细胞系的建立。
Establishment of an immortalized chondrocyte cell line with chondrocyte phenotype derived from rabbit mandibular condyle.
端粒酶激活可以维持增殖细胞染色体的稳定性，导致细胞永生化和肿瘤发生。
Telomerase activation is essential for the stabilization of chromosomes in proliferation cells, leading to cellular immortality and oncogenesis.
潜伏或永生化感染可以在DNA病毒感染的细胞中引起，因其限制或缺乏病毒基因转录所需的设备材料。
A latent or immortalizing infection may result from DNA virus infection of a cell that restricts or lacks the machinery for transcribing all the viral genes.
粒子诱发人胎永生化气管成纤维细胞体外恶性转化细胞生物学特征研究。
The study of cell biological character in the neoplastic transformation of immortalizational human fetal tracheal fibroblasts induced by alpha-particle irradiation.
目的建立永生化的人成骨细胞系供骨科临床和基础研究使用。
Objective To establish an immortalized osteoblast cell line used for basal and clinical research of orthopaedics.
说明该永生化胚胎肝细胞可以成为生物人工肝及肝细胞移植研究中的理想细胞材料。
It is concluded that the immortalized human fetal hepatocytes would become i-deal cell material on the study of bioartificial liver and hepatocytes transplantation.
端粒酶是一种RNA依赖的DNA多聚酶，可以合成端粒dna的重复片段与细胞永生化密切相关。
Telomerase is RNA dependent DNA polymerase, ACTS to synthesize DNA repeat fragment of telomeres, which is associated with immortalization of cells.
从而建立了永生化的人骨髓间充质干细胞。
Thereby establishing the immortalization of human mesenchymal stem cells.
目的：构建永生化肝细胞系并对其生物学特性及某些功能进行研究。
AIM: to establish an immortalized human fetal hepatocyte line and to study its biological characteristics and functions.
目的构建携带双自杀基因且可诱导敲除SV40T的逆转录病毒载体，优化目前的肝细胞永生化。
Objective To construct a new retroviral vector with double-suicide genes and knockout-inducible SV40T gene, with the aim of optimizing immortalization of hepatocytes.
构建永生化肝细胞系并对其生物学特性及某些功能进行研究。
To establish an immortalized human fetal hepatocyte line and to study its biological characteristics and functions.
观察了烟草特异亚硝胺nnk诱发HPV -18永生化人类支气管上皮细胞(BEP2D)恶性转化过程中细胞生物学特征的变化。
The malignant transformation of HPV 18 immortalized human bronchial epithelial cell (BEP2D) induced by tobacco specific nitrosamine NNK and its biological characteristics were investigated.
目的探讨SV40如何介导细胞永生化，与细胞永生化现象相关的因素及它们的作用。
Objective to explore the SV40-mediated immortalization, the related factors and their roles in cell immortalization.
同时以端粒酶阳性的永生化人胚肾成纤维细胞HEK293作为阳性对照，以端粒酶阴性的人胚肺成纤维细胞HELF作为阴性对照。
Immortalized human embryonic kidney cell line HEK293 and the human embryonic lung fibroblasts HELF were used as positive control and negative control, respectively.
结论：肝窦状内皮细胞在体外培养条件下自发永生化。
Conclusions: in vitro sinusoid endothelial cells could spontaneously immortalize. Some biological characteristics of immortalized cells changed.
结果显示永生化胚胎肝细胞克隆形成率为31.2%，染色体核型分析表明细胞核型无明显异常，软琼脂集落形成试验表明细胞在软琼脂中不能生长。
It is showed that the cell clone-forming rat was 31. 2%. The immortalized human fetal hepatocytes had a normal karyotype and were not able to grow in soft AGAR culture.
以SV4 0大T抗原转化原代大鼠原代胶质细胞得到的永生化胶质细胞（RGLT）可解决这些问题。
Therefore, an immortalized rat glial cell line (RGLT) was established by SV40 large T-antigen (LTag) gene from the primary rat fetal glial cells.
肝细胞的理想来源为永生化人肝细胞和人干细胞，但要做到成功地应用于生物人工肝则对这两种细胞的功能及安全性均应加以改进。
The optimal source for liver cells are immortalized human hepatocytes and human stem cells. However, both functionality and safety of these cells should be improve…
肝细胞的理想来源为永生化人肝细胞和人干细胞，但要做到成功地应用于生物人工肝则对这两种细胞的功能及安全性均应加以改进。
The optimal source for liver cells are immortalized human hepatocytes and human stem cells. However, both functionality and safety of these cells should be improve…
应用推荐