目的：探讨血液透析患者血清和外周血单个核细胞(PBMC)中输血传播病毒(TTV)检测状况及意义。
Objective: To study the situation and significance of transfusion transmitted virus (TTV) in serum and peripheral blood mononuclear cell (PBMC) of hemodialysis patients.
目的检测白细胞介素-2（IL-2）联合IL-12诱导人外周血单个核细胞（PBMC）体外抗肿瘤作用。
Objective To examine the in vitro antitumor activity of normal human peripheral blood mono-nuclear cells(PBMC) induced by interleukin-2(IL-2) and IL-12.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
在2 9例供血员的外周血单个核细胞（MNC）均未检测到WT1基因表达，但在2 1例非恶性疾病患者骨髓MNC中有8例呈WT1基因阳性（38.1% ）。
None was WT1 positive in peripheral blood mononuclear cells (MNC) from 29 blood donors, while bone marrow MNCs from eight of 21 cases (38.1%) of nonmalignant patient WT1 gene expression were found.
在2 9例供血员的外周血单个核细胞（MNC）均未检测到WT1基因表达，但在2 1例非恶性疾病患者骨髓MNC中有8例呈WT1基因阳性（38.1% ）。
None was WT1 positive in peripheral blood mononuclear cells (MNC) from 29 blood donors, while bone marrow MNCs from eight of 21 cases (38.1%) of nonmalignant patient WT1 gene expression were found.
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