目的改良人源杀菌肽LL-37的氨基酸序列，提高其杀菌力，并在原核细菌中表达。
Objective To reconstruct the proteinic sequence of human cathelicidin LL-37 to increase the bactericidal activity of LL-37 and to express the reconstructed LL-37 (rLL-37) in bacterium.
核苷酸替换偏好性受其相邻碱基组成的影响现象已经在核基因组假基因序列和叶绿体基因组的非编码序列和编码序列中发现。
Influence of neighboring base composition on nucleotide substitution bias was observed in the sequence of nuclear pseudogenes and chloroplast non-coding and coding sequences.
核定位序列（NLS）是信号蛋白分子上与入核转运相关的氨基酸序列。
Nuclear localization sequence(NLS) is the specific amino acid sequence on the signaling proteins to be sufficient for their nuclear translocation.
目的：构建血管基膜衍生多功能肽克隆和原核表达载体，并对血管基膜衍生多功能肽氨基酸序列进行空间结构分析预测。
Objective: to construct cloning and prokaryotic expression vector of vascular basement membrane-derived multifunctional peptide and to analyze its space conformation.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
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