目的探讨本地区性传播疾病（STD）患者沙眼衣原体（CT）、解脲支原体（UU）的感染现状及使用核酸扩增杂交梳法检测CT、UU的价值。
Objective To detect the status of CT and UU infection in local (STD)patients, and the value of measuring CT and UU with Nucleic Acid Amplification Hybridization Climb method.
禽流感的检测大体可以分血清学检测和依赖于PCR技术的分子生物学检测，其中依赖核酸的扩增检测技术属于新型检测手段。
The methods of detecting AI include mainly serum-testing and molecular biologic methods depending on PCR, of which nucleic acid testing techniques depending PCR belongs to new detection methods.
结论利用肽核酸生物传感器成功地绕过了PCR扩增而直接检测出了临床标本中的HBV基因组dna。
CONCLUSIONS HBV DNA extracted from clinical samples were directly detected using PNA biosensor and PCR amplification was successfully bypassed.
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
这种方法不仅可以直接扩增DNA和RNA，还可以实现对靶核酸的信号放大，灵敏度达到一个拷贝的核酸分子，因此在核酸检测中具有很大的应用价值和潜力。
This method can not only amplify DNA and RNA directly, but also allow the enlargement of signal from target nucleic acids with the sensitivity up to one copy of nucleic acids molecule.
这种方法不仅可以直接扩增DNA和RNA，还可以实现对靶核酸的信号放大，灵敏度达到一个拷贝的核酸分子，因此在核酸检测中具有很大的应用价值和潜力。
This method can not only amplify DNA and RNA directly, but also allow the enlargement of signal from target nucleic acids with the sensitivity up to one copy of nucleic acids molecule.
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