方法:人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。
METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.
在腺病毒载体的构建过程中,早期和晚期启动子的选择、构建以及去除末端蛋白(TP)、克隆末端序列是至关重要的。
The selection and cloning of early and late promoters and removal of adenovirus terminal protein (TP) are very important in construction of adenovirus vector.
这种物质一般从小牛的胃中采集而来,里面含有的多种酶能够切断牛奶中的一种蛋白——酪蛋白的水溶性末端。
Then they add a substance usually harvested from veal stomachs called rennet, a mixture of enzymes that slice the ends off caseins, a kind of milk protein.
在每一个片段的末端,研究者连上一个荧光蛋白,在另一端他们工程DNA能熄灭荧光的亮度。
At one end of each snippet, the researchers attached a fluorescent protein, and, at the other end, they engineered the DNA so that it could "quench" the fluorescent light.
该单克隆抗体通过用合成肽免疫动物制备,该合成肽与人钙网蛋白靠近氨基末端的残基一样。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human calreticulin protein.
单克隆抗体由合成的肽段免疫动物产生。其合成的肽段与邻近人源gata - 6蛋白的氨基末端氨基酸残基序列一致。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-6 protein.
此多克隆抗体经合成与人usp14蛋白羧基末端邻近氨基酸残基序列一致的肽段,免疫动物产生。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human USP14 protein.
该单克隆抗体是经合成与人usp10蛋白氨基末端邻近氨基酸残基序列一致的肽段,免疫动物产生。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human USP10 protein.
通过免疫组织化学方法和原位末端标记法(TUNEL)检测热休克蛋白70表达及凋亡细胞数,TTC染色观察梗死体积。
The expression of heat shock protein 70 was examined by immunohistochemistry, the apoptosis of neurons by TUNEL and the volume of cerebral ischemia by TTC staining.
此单克隆抗体由合成肽段免疫动物产生,该肽段与人ndrg1蛋白邻近羧基末端氨基酸残基序列一致。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NDRG1 protein.
但是现在我们知道了这个末端可以调节蛋白的形成并且在肿瘤中起作用。
But now we know that this end often has a protein production regulatory program and in some cases can play a role in cancer.
该单克隆抗体由合成的人类EGR1蛋白氨基末端附近肽段免疫动物而制成。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the sequence of human EGR1.
此单克隆抗体由合成肽段免疫动物产生,该肽段与人gata - 3蛋白氨基末端邻近氨基酸残基序列一致。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-3 protein.
的蛋白质新陈代谢的末端不有益于关节炎滑囊炎和骨关节炎尿。
The end of protein metabolism is uric which is not good for arthritis bursitis and osteoarthritis.
多克隆抗体采用人f KBP5蛋白羧基末端残基相对应的合成肽段免疫动物生产的。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FKBP5 protein.
该单克隆抗体是通过用重组蛋白免疫动物而制备的,该重组蛋白是人FGF受体2蛋白羧基末端区域的残基。
Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to residues near the carboxy terminal region of human FGF receptor 2 protein.
氨肽酶是一类从多肽链的氮末端顺序逐个水解氨基酸的酶,在蛋白水解液脱苦和蛋白质的深度水解中有重要作用。
Aminopeptidase, a collection of enzymes which can remove amino acid from the N-terminus of peptides one by one, is very important in protein hydrolysate production and debittering.
根瘤菌特异性地结合在根须末端,粘附在一种叫做凝集素的特殊植物蛋白之后进入根须。
The rhizobia bind specifically to the root hair tip, attaching to special plant proteins called lectins.
先前世界范围内数个实验室的组织培养研究揭示胡乱的修改末端将阻止这个蛋白降解或激活。
Previous tissue culture studies by several LABS around the world indicated that tinkering with the tail end prevented the protein from being flagged for degradation or activation.
目的:对牛牙骨质附着蛋白进行N末端氨基酸序列分析,为牙骨质附着蛋白的克隆表达提供一定的理论依据。
Objective:To analyze the N-terminal amino acid sequence of bovine cementum attachment protein(CAP), which lay a theoretical foundation for cloning and expression of the CAP.
该单克隆抗体通过合成肽免疫动物制备,这种合成肽与LC3蛋白氨基末端周围残基相一致。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of LC3B.
该单克隆抗体是通过用重组蛋白免疫动物而制备的,该重组蛋白是人FGF受体1蛋白的羧基末端。
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein.
该单克隆抗体由人类CREB蛋白氨基末端附近的氨基酸序列对应的重组蛋白免疫动物而制成。
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CREB protein.
该单克隆抗体是通过用重组蛋白免疫动物而制备的,该重组蛋白是针对人FGF受体1蛋白的羧基末端。
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein.
该单克隆抗体是通过用重组蛋白免疫动物而制备的,该重组蛋白包含人VEGF受体2蛋白羧基末端150氨基酸残基。
Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 protein.
该单克隆抗体是采用合成的人FIP200蛋白羧基末端附近的残基相对应的肽段免疫动物制备。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FIP200 protein.
该单克隆抗体是由针对人TACC3蛋白近氨基末端残基的肽段免疫动物生产的。
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TACC3 protein.
研究人员发现,端粒中的RNA受端粒末端酶中的一种蛋白调节,这一发现揭开了端粒功能的关键问题。
The researchers discovered that the RNA in the telomere is regulated by a protein in the telomerase enzyme. Their discovery may thus uncover key elements of telomere function.
染尘肌体组织在加热过程中,蛋白质类结构对称性降低并逐渐氧化,末端基团如—ch_3,—CH_2振动减弱。
When injected organism tissues were heated, the protein structural symmetry was reduced, oxidated gradually, and the vibration weakened at the end radicel such as -ch3, -ch2.
这种酶修复并加长了端粒——对免疫力和寿命来说至关重要的染色体末端的微小蛋白质复合物。
This enzyme repairs and lengthens telomeres, tiny protein complexes on the ends of chromosomes that are vital for immunity and longevity.
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