探讨应用活细胞荧光染料CFSE 检测T淋巴细胞各亚群增殖反应的方法学。
Evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using carboxyfluorescein diacetate succinimidyl ester labeling assay.
CFSE标记是一种有效的检测淋巴细胞增殖的方法。
CFSE staining is an effective method for lymphocyte proliferative assay.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
采用MTT方法检测淋巴细胞增殖反应。
MTT method was used to determine the proliferation of lymphocyte.
方法：应用MTT法观察体外壳寡糖和GAH对小鼠脾淋巴细胞细胞增殖的影响，应用巨噬细胞吞噬中性红试验观察壳寡糖和GAH对小鼠腹腔巨噬细胞吞噬功能的影响。
Methods:To observe the effects of the Chitooligosaccharidean and GAH on the lymphocyte proliferation of mouse by MTT assay and the phagocytosis of macrophages by Tuluylene red pinocyfic test.
方法应用流式细胞术检测了35例急性淋巴细胞性白血病患者骨髓细胞DNA含量(DI)、倍体和细胞增殖活性(PI)。
Methods The DNA index (DI) and proliferation index (PI) in aspirated myeloid cells were determinated in 35 patients with ALL by flow cytometry (FCM).
方法：用10 0 %当归水浸出液定期给小鼠灌胃，然后检测小鼠淋巴细胞增殖反应。
Methods:Filled the stomachs of the mice with 100% water extract of AM regularly, then exposed them to X-ray radiation and tested lymphocyte cells proliferation.
方法：用10 0 %当归水浸出液定期给小鼠灌胃，然后检测小鼠淋巴细胞增殖反应。
Methods:Filled the stomachs of the mice with 100% water extract of AM regularly, then exposed them to X-ray radiation and tested lymphocyte cells proliferation.
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