对复性后的单链抗体以抗原捕获ELISA法进行检测。
The refolded single-chain antibodies were examined by antigen capture ELISA.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
以人疱疹病毒为抗原，用酶免疫法（EIA）比较在不同生境中，红面猴B病毒相关抗体阳性率的差异：野外捕获组阳性率为76.2 % ；
The related antibody to virus(BVRA) of M. arctoides , which were from, the different habitat, was detected by EIA using HSV as antigen. The Positive rate of the wild captive group was 76.2%;
以人疱疹病毒为抗原，用酶免疫法（EIA）比较在不同生境中，红面猴B病毒相关抗体阳性率的差异：野外捕获组阳性率为76.2 % ；
The related antibody to virus(BVRA) of M. arctoides , which were from, the different habitat, was detected by EIA using HSV as antigen. The Positive rate of the wild captive group was 76.2%;
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